지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.3
- 수록면
- 5 - 9 (5page)
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It has been shown that the accumulation of prion in the cytoplasm can result in neurodegenerative disorders. Synthetic prion peptide 106-126 (PrP) is a glycoprotein that is expressed predominantly by neurons and other cells, including glial cells. Prion-induced chronic neurodegeneration has a substantial inflammatory component, and an increase in the levels of matrix metalloproteinases (MMPs) may play an important role in neurodegenerative development and progression. However, the expression of MMPs in PrP induced rat astrocytes and microglia has not yet been compared. Thus, in this study, we examined the fluorescence intensity of CD11b positive microglia and Glial Fibrillary Acidic Protein (GFAP) positive astrocytes and found that the fluorescent intensity was increased following incubation with PrP at 24 hours in a dose-dependent manner. We also observed an increase in interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) protein expression, which are initial inflammatory cytokines, in both PrP induced astrocytes and microglia. Furthermore, an increase MMP-1, 3 and 11 expressions in PrP induced astrocytes and microglia was observed by real time PCR. Our results demonstrated PrP induced activation of astrocytes and microglia respectively, which resulted in an increase in inflammatory cytokines and MMPs expression. These results provide the insight into the different sensitivities of glial cells to PrP.
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REFERENCES
참고문헌 (19)
참고문헌 신청
Betmouni, S. / 1996 / Evidence for an early inflammatory response in the central nervous system of mice with scrapie / Neuroscience 74:1~5
손효진 / 200807 / A novel intracellular role of matrix metalloproteinase-3 during apoptosis of dopaminergic cells / JOURNAL OF NEUROCHEMISTRY 106:405~415
Crocker, S.J / 2008 / A novel method to establish microglia-free astrocyte cultures: Comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia / Glia 56:1187~1198
Crocker, S. J. / 2006 / Cell and agonist-specific regulation of genes for matrixmetalloproteinases and their tissue inhibitors by primary glialcells / J. Neurochem 98:812~823
Crocker, S. J. / 2004 / The TIMPs tango with MMPs and more in the central nervous system / J. Neurosci. Res 75:1~11
손효진 / 200807 / A novel intracellular role of matrix metalloproteinase-3 during apoptosis of dopaminergic cells / JOURNAL OF NEUROCHEMISTRY 106:405~415
Crocker, S.J / 2008 / A novel method to establish microglia-free astrocyte cultures: Comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia / Glia 56:1187~1198
Crocker, S. J. / 2006 / Cell and agonist-specific regulation of genes for matrixmetalloproteinases and their tissue inhibitors by primary glialcells / J. Neurochem 98:812~823
Crocker, S. J. / 2004 / The TIMPs tango with MMPs and more in the central nervous system / J. Neurosci. Res 75:1~11
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UCI(KEPA) : I410-ECN-0101-2013-513-001729956