지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.3
- 수록면
- 55 - 60 (6page)
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초록· 키워드
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Effects of egg york containing IgY specific for Helicobacter pylori on the bacterial growth and intragastric infection were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro antibacterial activity test, H. pylori (1×108 CFU/mL) was incubated with a serially diluted IgY for 3 days. As a result, IgY fully inhibited the bacterial growth at 16 mg/mL, which was determined to a minimal inhibitory concentration. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1×108 CFU/mouse) 3 times at 2-day intervals, and 2 weeks later, orally treated twice a day with 50, 100, 200 or 500 mg/kg IgY for 18 days. After the final administration, biopsy sample of the gastric mucosa was assayed for the bacterial identification via urease, oxidase, catalase, nitrate reduction and H2S tests in addition to microscopic examination for mucosal inflammation. In CLO kit test, 75, 50, 12.5 and 12.5% of the animals revealed positive reaction following treatment with 50, 100, 200 and 500 mg/kg IgY, respectively, resulting in a superior efficacy at 200 mg/kg than 30 mg/kg pantoprazole that displayed 75% elimination. The CLO test results were confirmed by bacterial identification. Microscopic examination revealed that H. pylori infection caused severe gastric mucosal inflammation, which were not observed in the CLO-negative mice following treatment with IgY or pantoprazole. Taken together, IgY inhibited the growth of H. pylori, and improved gastritis and villi injuries by eliminating the bacteria from the stomach. The results indicate that IgY could be a good candidate overcoming tolerance of antibiotics for the treatment of H. pylori-mediated gastric ulcers.
목차
Materials and Methods
Results
Discussion
Acknowledgments
References
참고문헌 (42)
참고문헌 신청
Wallace JL / 1996 / The cellular and molecular basis of gastric mucosal defense / FASEB J 10(7):731~740
Neal MJ / 2003 / Medical Pharmacology at a Glance. 3rd ed / Blackwell Publishing Inc :30~31
Isobe H / 2004 / Activated protein C reduces stress-induced gastric mucosal injury in rats by inhibiting the endothelial cell injury / J Thromb Haemost 2(2):313~320
Yun-Bae Kim / 2007 / The Role of Corticosteroids in Stress-Induced Gastric Ulceration in Rats / Laboratory Animal Research 23(2):127~131
Slomiany BL / 1997 / Induction of tumor necrosis factor-alpha and apoptosis in gastric mucosal injury by indomethacin: effect of omeprazole and ebrotidine / Scand J Gastroenterol 32(7):638~642
Neal MJ / 2003 / Medical Pharmacology at a Glance. 3rd ed / Blackwell Publishing Inc :30~31
Isobe H / 2004 / Activated protein C reduces stress-induced gastric mucosal injury in rats by inhibiting the endothelial cell injury / J Thromb Haemost 2(2):313~320
Yun-Bae Kim / 2007 / The Role of Corticosteroids in Stress-Induced Gastric Ulceration in Rats / Laboratory Animal Research 23(2):127~131
Slomiany BL / 1997 / Induction of tumor necrosis factor-alpha and apoptosis in gastric mucosal injury by indomethacin: effect of omeprazole and ebrotidine / Scand J Gastroenterol 32(7):638~642
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UCI(KEPA) : I410-ECN-0101-2013-510-001698946