Purpose: The study was to identify the relationships between levels of knowledge, attitude and preventive health behavior about cancer in university students. Methods: The data were analyzed with the SPSS/Win 18.0 program. The participants of this study were 200 university students. Three kinds of instruments were used for this study, questionnaires about cancer-related knowledge, attitude and preventive health behavior from Suh et al. (1998). Results: The mean score for knowledge about cancer was 14.33±5.32, the mean score of attitude toward cancer was 31.76±3.75 and the mean score of preventive behavior for cancer was 57.20±8.48. There was slightly positive correlation between knowledge of cancer and attitude toward cancer. There was positive correlation between the attitude toward cancer and preventive health behavior for cancer. Conclusion: It is necessary to consider the related factors for the development and implementation of systematic education programs that can encourage and promote preventive health behavior for cancer among university students.