The purpose of this study was to investigate leisure motivation scale construct validity and to verify change of factor pattern with gender using multi-group factor invariance analysis. To carry out this research, used 485 university students(male 304, female 181). The data from this study were analyzed using SPSS 15.0 for common factor analysis of exploratory factor analysis; using AMOS 7.0 for confirmatory factor analysis and chi-square test of multi-group analysis. The results of this study were as following; first, the configure, metric, partial scalar invariance were satisfied for multi-group confirmatory factor analysis. Second, according to the latent mean analysis, female group tended to have higher levels than male group(intrinsic motivation latent mean was .15, p<.001). Third, when it comes to effect size; intrinsic motivation factor .40, introjected regulation factor .17, identified regulation factor .12, and amotivation factor was -.15.