지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 471 - 478 (8page)
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초록· 키워드
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Ginseng, the root of Panax ginseng, is one of the oldest herbal medicines. It has a variety of physiological and pharmacological effects. Recently, we isolated a subset of glycolipoproteins that we designated gintonin, and demonstrated that it induced transient change in intracellular calcium concentration ([Ca<SUP>2+</SUP>]<SUB>i</SUB>) in cells via G-protein-coupled receptor signaling pathway(s). The previous method for gintonin isolation included multiple steps using methanol, butanol, and other organic solvents. In the present study, we developed a much simple method for the preparation of gintonin from ginseng root using 80% ethanol extraction. The extracted fraction was designated edible gintonin. This method produced a high yield of gintonin (0.20%). The chemical characteristics of gintonin such as molecular weight and the composition of the extract product were almost identical as the gintonin prepared using the previous extraction regimen involving various organic solvents. We also examined the physiological effects of edible gintonin on endogenous Ca<SUP>2+</SUP>-activated CI" channel activity of Xenopus oocytes. The 50% effective dose was 1.03±0.3 ug/ml., Finally, since gintonin preparation through ethanol extraction is easily reproducible, gintonin could be commercially applied for ginseng-derived functional health food and/or drug following the confirmations of in vitro and in vivo physiological and pharmacological effects of gintonin.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (10)
참고문헌 신청
1. [학술지(정기간행물)] - Nah SY / 2007 / Ginsenosides: are any of them candidates for drugs acting on the central nervous system? / CNS Drug Rev 13 : 381 ~ 404
2. [학술지(정기간행물)] - / 2001 / G alpha(q/11) coupled to mammalian phospholipase C beta 3-like enzyme mediates the ginsenoside effect on Ca2+-activated Cl- current in the Xenopus oocyte / JOURNAL OF BIOLOGICAL CHEMISTRY 276 : 48797 ~ 48802
3. [학술지(정기간행물)] - Choi S / 2001 / A novel activation of Ca(2+)-activated Cl(-) channel in Xenopus oocytes by Ginseng saponins: evidence for the involvement of phospholipase C and intracellular Ca(2+) mobilization / Br J Pharmacol 132 : 641 ~ 648
4. [학술지(정기간행물)] - Lee JH / 2004 / Prevention of ginsenoside-induced desensitization of Ca2+-activated Cl- current by microinjection of inositol hexakisphosphate in Xenopus laevis oocytes - Involvement of GRK2 AND beta-arrestin I / JOURNAL OF BIOLOGICAL CHEMISTRY 279 (11) : 9912 ~ 9921
6. [학술지(정기간행물)] - Mi Kyung Pyo / 2011 / A Simple Method for the Preparation of Crude Gintonin from Ginseng Root, Stem, and Leaf / Journal of Ginseng Research 35 (2) : 209 ~ 218
2. [학술지(정기간행물)] - / 2001 / G alpha(q/11) coupled to mammalian phospholipase C beta 3-like enzyme mediates the ginsenoside effect on Ca2+-activated Cl- current in the Xenopus oocyte / JOURNAL OF BIOLOGICAL CHEMISTRY 276 : 48797 ~ 48802
3. [학술지(정기간행물)] - Choi S / 2001 / A novel activation of Ca(2+)-activated Cl(-) channel in Xenopus oocytes by Ginseng saponins: evidence for the involvement of phospholipase C and intracellular Ca(2+) mobilization / Br J Pharmacol 132 : 641 ~ 648
4. [학술지(정기간행물)] - Lee JH / 2004 / Prevention of ginsenoside-induced desensitization of Ca2+-activated Cl- current by microinjection of inositol hexakisphosphate in Xenopus laevis oocytes - Involvement of GRK2 AND beta-arrestin I / JOURNAL OF BIOLOGICAL CHEMISTRY 279 (11) : 9912 ~ 9921
6. [학술지(정기간행물)] - Mi Kyung Pyo / 2011 / A Simple Method for the Preparation of Crude Gintonin from Ginseng Root, Stem, and Leaf / Journal of Ginseng Research 35 (2) : 209 ~ 218
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UCI(KEPA) : I410-ECN-0101-2013-524-001212655