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논문 기본 정보

자료유형
학술저널
저자정보
이종선 (경희대학교) 조윤희 (경희대학교)
저널정보
한국양봉학회 Journal of Apiculture 한국양봉학회지 제26권 제4호
발행연도
2011.12
수록면
297 - 305 (9page)

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Epidermal hydration is maintained by epidermal lipid barrier, in which sphingolipids are major lipids. Of various sphingolipids, glucosylceramides (GlcCer) and sphingomyelins (SM) are hydrolyzed to generate various ceramide (Cer) species, of which Cer 1 plays a role in maintaining epidermal barrier. We examined whether dietary supplementation of royal jelly (RJ) during pre and post menopausal period alters epidermal levels of hydration as well as of sphingolipids and their metabolizing enzymes. SD rats were divided into five groups: groups SHAM and OVX (ovariectomy) with control diet, groups RJ1 and RJ2: ovariectomized rats with diet containing 1% 1 or 2 RJ from Korea, group IF: ovariectomized rats with diet containing 80mg/kg isoflavone. Ovariectomy was performed at 4wk of diet supplementation, which was continued for 8 more wks. After 12wks, with no difference of food effici-ency, epidermal levels of hydration and total Cer did not differ in all groups. However, despite of higher expression of acid sphing omyelinase (aSMase), an enzyme of SM hydrolysis into Cer, epidermal levels of Cer1, as well as of total GlcCer and SM in group OVX were significantly lowered than in group SHAM, and those of groups RJ2 and IF was similar to in group SHAM. Notably, epidermal levels of GlcCer, SM and Cer1 in group RJ1 were significantly higher than in group OVX, which is paralleled with an increased protein expression of β-glucocerebrosidase (β-GlcCer’ase), an enzyme of GlcCer hydrolysis into Cer. Dietary RJ1 enhances the epidermal levels of GlcCer, SM and Cer1 coupled with an increased protein expression of β-GlcCer’ase.

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UCI(KEPA) : I410-ECN-0101-2013-527-001169498