Black queen cell virus (BQCV) causes the death of queen larvae and pupae. The coding sequence of structural protein gene in BQCV was amplified using cDNA from Apis mellifera in Korea and cloned into the pQE30-ELP (Elastin-like Polypeptide) vector at BamHI/SalI restriction enzyme site. It was presented 99% nucleotide and 100% amino acid sequence identification with BQCV sequences reported (GenBank AF183905). pQE30-BQCV-ELP was expressed in Escherichia coli M15. pQE30-BQCV in M15 was successfully expressed in the state of more than 0.1mM IPTG. This recombinant BQCV protein will be use for generation of monoclonal antibody to develop a diagnosis tool for BQCV infection in honeybee.