지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 267 - 274 (8page)
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Sacbrood virus (SBV) is an infectious disease which affects the brood of honeybees, resulting in failure to pupate and death. A loop-mediated isothermal amplification (LAMP) assay allows one-step detection of gene amplification without expensive equipment such as thermocycler. The SBV-LAMP method was developed for detection of SBV easily and rapidly. A set of four designed primers named SBV-F3/B3/FIP/BIP that recognize SBV specific sequence. After optimization of conditions, SBVspecific amplification was successfully performed using SBV-LAMP from standard. SBV-specific template under isothermal conditions at 57°C within 60 minutes. The method could be detected SBV in DNA clone at 103copies/μl, also could be applied not only cDNA but also field samples. Especially, this method was developed direct detection analysis by the naked eye using SYBR Green I, Gene-FinderTM Nucleic acid fluorescent dye and phenol red. SBV-LAMP may be expected to be useful for the specific detection of SBV in field and for the monito ring of natural infection in Apis mellifera L. by SBV.
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UCI(KEPA) : I410-ECN-0101-2013-527-001169463