지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 247 - 251 (5page)
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초록· 키워드
오류제보하기
To clarify whether inhibitory effect of estrogen on liver tumor is associated with cell proliferation, we investigated its role in diethylnitrosamine (DEN)-induced rat preneoplastic lesions, with time sequenced manners. F344 male rats (n = 90) were divided into three groups at 5 weeks of age. The mini-osmotic pumps providing a continuous infusion of DEN was implanted into the abdominal cavity of each animal in group 1, 2 and 3 at 6 weeks of age. To see the effect of estrogen, pellet containing 1 or 10μg of estradiol-3-benzoate (EB) was implanted subcutaneously in the animals of groups 2 or 3, respectively, one week prior to DEN treatment. Ten animals of each group were euthanized at 10, 14 and 18 weeks after DEN treatment. Liver tissues at each time point were fixed in 10% phosphate-buffered formalin and were processed and embedded in paraffin and 5μm sections mounted on a silanized slide. Glutathione S-transferase placental form (GST-P) positive foci and 5-bromo-2-deoxyuridine (BrdU) labeling cells were detected at each time point. Area of GST-P positive foci in DEN+EB 1 or 10μg group was significantly decreased compared to DEN alone at 14 weeks (p < 0.01 or p < 0.05, respectively) an at 18 weeks (p< 0.05 or p< 0.01, respectively). BrdU index in DEN+EB 1 or 10μg groups was significantly decreased compared to DEN alone at 14 weeks and at 18 weeks (p < 0.01). Taken together, we conclude that EB treatment decrease the DEN-induced liver preneoplastic lesions and this may be associated with decrease of cellular proliferation.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (25)
참고문헌 신청
Bosch, F.X. / 1999 / Epidemiology of primaryliver cancer / Semin. Liver. Dis 19:271~285
Cover, C.M. / 1999 / Indole-3-carbinoland tamoxifen cooperate to arrest the cell cycle of MCF-7human breast cancer cells / Cancer Res 59:1244~1251
Cover, C.M. / 1998 / Indole-3-carbinolinhibits the expression of cyclin-dependent kinase-6 andinduces a G1 cell cycle arrest of human breast cancer cells independentof estrogen receptor signaling / J. Biol. Chem 273:3838~3847
Dashwood, R.H. / 1989 / Quantitative inter-relationshipsbetween aflatoxin B1 carcinogen dose,indole-3-carbinolanti-carcinogen dose,target organ DNA adduction and finaltumor response / Carcinogenesis 10:175~181
De Maria, N. / 2002 / Sex hormones andliver cancer / Mol. Cell Endocrinol 193:59~63
Cover, C.M. / 1999 / Indole-3-carbinoland tamoxifen cooperate to arrest the cell cycle of MCF-7human breast cancer cells / Cancer Res 59:1244~1251
Cover, C.M. / 1998 / Indole-3-carbinolinhibits the expression of cyclin-dependent kinase-6 andinduces a G1 cell cycle arrest of human breast cancer cells independentof estrogen receptor signaling / J. Biol. Chem 273:3838~3847
Dashwood, R.H. / 1989 / Quantitative inter-relationshipsbetween aflatoxin B1 carcinogen dose,indole-3-carbinolanti-carcinogen dose,target organ DNA adduction and finaltumor response / Carcinogenesis 10:175~181
De Maria, N. / 2002 / Sex hormones andliver cancer / Mol. Cell Endocrinol 193:59~63
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UCI(KEPA) : I410-ECN-0101-2013-513-001112920