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논문 기본 정보

자료유형
학술저널
저자정보
Sang-Hoon Han (농촌진흥청 국립농업과학원) Yong-Soo Choi (국립농업과학원) Myeong-Lyeol Lee (농촌진흥청 국립농업과학원)
저널정보
한국양봉학회 Journal of Apiculture 한국양봉학회지 제26권 제3호
발행연도
2011.10
수록면
233 - 240 (8page)

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초록· 키워드

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Recently, widespread infection of Korean Sacbrood virus (KSBV) caused a great loss of Korean honeybee (Apis cerana) colonies. There were several reports for the development of a PCR based detection method of SBV. These detection primer sets, however, produced a low limit of detection (LOD), false positive, or unspecific amplicons in some of cases. For these reasons, a development of a highly specific and sensitive detection primer set is required for a rapid and accurate detection of KSBV genetic materials. In this study, a specific primer set, SBV-C147-F/R was designed to detect a KSBV-specific genomic region. It repeatedly produced 147bp PCR amplicons with cDNAs from KSBV-infected honeybee larvae. In quantitative real-time PCR assays, the primer set could detect 8.7 copy of pSBV which is a recombinant plasmid including a partial KSBV genomic fragment (GenBank Accession HQ322114: 3043-3189). In comparison with previously reported four primer sets for the detection of SBV, CSBV and KSBV, SBV-C147 showed the most highest sensitivity to identify KSBVinfected larvae among environmental larval honeybee samples. In conclusion, SBV-C147 primer set is expected to be a reliable diagnostic tool for KSBV infection and may be a useful means of an early quarantine for KSBV infection in Korean apiaries rearing A. cerana.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
ACKNOWLEDGEMENTS
LITERATURE CITED

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UCI(KEPA) : I410-ECN-0101-2013-527-000925023