지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.10
- 수록면
- 213 - 220 (8page)
이용수
초록· 키워드
오류제보하기
A novel loop-mediated isothermal amplification (LAMP)-based detection method was established for the rapid detection of Paenibacillus larvae (P. larvae), which is the causative agent of American foulbrood (AFB). This method enabled amplification of a part of the metalloproteinase (Mtpr) gene at a temperature of 63℃ within 45min, with no need for thermal cycling. The specificity of the method was demonstrated by successful amplification of the bee genome infected with P. larvae and by HindⅢ restriction enzyme analysis applied to the inner primers. The present method detected as low as 2 fg of DNA plasmid. Visual inspection of LAMP products using Gene finder demonstrated that the positive and negative reactions exhibited distinct and different colors in daylight to the naked eye without a UV illuminator or other supporting tools, which means that gel electrophoresis was not necessary to appreciate the presence of P. larvae. Therefore, this detection method is rapid, sensitive, specific, and low in cost and it may be used to facilitate development of apiaries.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
LITERATURE CITED
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Allipi, A.M. / 2002 / Differentiation of Paenibacillus larvae subsp. larvae, the cause of American Foulbrood in honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA / Applied and Environmental Microbiology 68 (7) : 3655 ~ 3660
2. [학술지(정기간행물)] - Antunez, K. / 2004 / Paenibacillus larvae spores in honey samples from Uruguay: a nationwide survey / Journal of Invertebrate Pathology 86 (1) : 56 ~ 58
3. [학술지(정기간행물)] - Bakonyi, T. / 2003 / Development and evaluation of PCR assays for the detection of Paenibacillus larvae in honey samples: comparison with isolation and biochemical characterization / Applied and Environmental Microbiology 69 (3) : 1504 ~ 1510
4. [학술지(정기간행물)] - Chagas, S.S. / 2010 / Detection of Paenibacillus larvae by Real-Time PCR / Acta Scientiae Veterinariae 38 (3) : 251 ~ 256
5. [학술지(정기간행물)] - Eva, F. / 2010 / Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae / Apidologie 41 : 99 ~ 108
2. [학술지(정기간행물)] - Antunez, K. / 2004 / Paenibacillus larvae spores in honey samples from Uruguay: a nationwide survey / Journal of Invertebrate Pathology 86 (1) : 56 ~ 58
3. [학술지(정기간행물)] - Bakonyi, T. / 2003 / Development and evaluation of PCR assays for the detection of Paenibacillus larvae in honey samples: comparison with isolation and biochemical characterization / Applied and Environmental Microbiology 69 (3) : 1504 ~ 1510
4. [학술지(정기간행물)] - Chagas, S.S. / 2010 / Detection of Paenibacillus larvae by Real-Time PCR / Acta Scientiae Veterinariae 38 (3) : 251 ~ 256
5. [학술지(정기간행물)] - Eva, F. / 2010 / Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae / Apidologie 41 : 99 ~ 108
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UCI(KEPA) : I410-ECN-0101-2013-527-000925058