Radiation exposure may cause tissue damage by ionized or excited atoms. The molecules are transformed from water or they directly interact with cells. Antioxidants such as vitamin E, vitamin C are used to reduce the interference of radiation. Especially, vitamin E inhibits the formation and accumulation of lipid and oxidation and the activity of drug-metabolizing enzymes. After irradiation of 3 Gy to the testis, the entire shape of testis was partly depressed and an irregular vacuole was observed to the edge. The 3 Gy group after treatment with vitamin E showed the low destruction of spermatogonia and partial tissue damage as compared to the 3 Gy group. Vitamin E inhibited the production of vacuoles from the cell death and generated the block to prevent the condensation of the seminiferous tubules.