지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 177 - 184 (8page)
이용수
초록· 키워드
오류제보하기
Potassium cyanate (KOCN) is an inorganic compound and induces the carbamylation of proteins with cytotoxic effects on human cells. Although there is a potential cytotoxic molecule, the role of KOCN on the apoptosis of cancer cell is not well understood. The present study investigated the effects of KOCN on the human colorectal cancer cell line, HCT 116 cells. To understand the anti-cancer effect of KOCN on HCT 116 cells, we examined alteration of apoptosis, the intracellular Ca<sup>2+</sup> concentration, the intracellular signaling pathway and generation of reactive oxygen species (ROS) in these cells treated with KOCN. The apoptosis of HCT 116 cells was induced by KOCN in a dose-dependent manner at 24 hours and 48 hours, respectively. The apoptosis was processed via the cleavage of poly ADP-ribose polymerase (PARP) and activation of caspase 3 in HCT 116 cells. KOCN induced the elevation of intracellular Ca<sup>2+</sup> concentration and changed the expressions of Bcl-2 family proteins. The pro-apoptotic Bax was continuously up-regulated, and the anti-apoptotic Bcl-2 was down-regulated by KOCN. KOCN also induced the hyperpolarization of mitochondria and the generation of ROS in HCT 116 cells. Taken together, these results indicate that KOCN induces the apoptosis of HCT 116 cells by disruption of Ca<sup>2+</sup> homeostasis and via mitochondrial pathway. This study provides the compound that may be used as a potent agent for the treatment of colorectal cancer.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (23)
참고문헌 신청
Apostolov EO / 2011 / Endonuclease G mediates endothelial cell death induced by carbamylated LDL / Am J Physiol Heart Circ Physiol 300:1997~2004
Clapham DE / 2007 / Calcium signaling / Cell 131:1047~1058
Cory S / 2002 / The Bcl-2 family: regulators of the cellular life-or-death switch / Mat Rev Cancer 2:647~656
Desagher S / 2000 / Mitochondria as the central control point of apoptosis / Trends Cell Biol 10:369~377
Espinoza LA / 2007 / Prolonged poly (ADPribose) polymerase-1 activity regulates JP-8-induced sustained cytokine expression in alveolar macrophages / Free radical biology & medicine 42:1430~1440
Clapham DE / 2007 / Calcium signaling / Cell 131:1047~1058
Cory S / 2002 / The Bcl-2 family: regulators of the cellular life-or-death switch / Mat Rev Cancer 2:647~656
Desagher S / 2000 / Mitochondria as the central control point of apoptosis / Trends Cell Biol 10:369~377
Espinoza LA / 2007 / Prolonged poly (ADPribose) polymerase-1 activity regulates JP-8-induced sustained cytokine expression in alveolar macrophages / Free radical biology & medicine 42:1430~1440
이 논문의 저자 정보
이 논문과 함께 이용한 논문
사람과 미래환경
강미아
한국환경정책학회 학술대회논문집
2016
.02
동아시아 생명과학의 발전과 바이오시민권의 형성가능성
김기흥
아시아리뷰
2012
.12
Effects of the Photic Stimulation on Electroencephalogram in Pediatric Epilepsy Patients
Joong Soo Yoon
,
Hyun Ju Choi
대한의생명과학회지
2012
.12
A Hydrogel Film Containing Propolis Nanoparticles as a Wound Healing Membrane
Jin Kim
,
Yong Moon Kim
,
Dong-Woon Kim
외 1명
대한의생명과학회지
2012
.06
Clinical Evaluation of Human Papillomavirus DNA Genotyping Assay to Diagnose Women Cervical Cancer
Sunghyun Kim
,
Dongsup Lee
,
Yeun Kim
외 8명
대한의생명과학회지
2012
.06
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2013-510-000796372