지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The objectives of this study are to identify specific functional genes which are related with growth and protein structure of the pectoral muscle of Korean native chicken. Pectoral muscle was isolated from three Korean native chickens (KNC, red brown, 12 months old, 2.41 ± 0.24 kg) and three Cornish chickens (16 month old, 2.76 ± 3.0 kg). The subtraction cDNA library was prepared in PCR4 Blunt-TOPO vector. The DNA sequence homology was compared with other breeds and species in GenBank. A clone NDS-81 was found to be unique for the DNA sequence homology with UBX family. Their partial sequence has high homology (98%) with chicken UBX domain D. Chicken UBX domain has chicken (93%), cattle (68%), dog (67%), mouse (64%) and, human (63%) nucleotide sequence homology. Several regions were mutated from T in chicken to C or G in the NDS-81 clone. The first site is LAD in chicken, but it was expressed as (L)RM in clone NDS-81. In this site, amino acids were changed from Ala to Arg, and from Asp to Met. The second site was changed from ER (Arg) in chicken to ED (Asp) in clone NDS-81. They are both containing functional side chains and play an important role in binding other proteins. Therefore, the clone NDS-81 could be a different candidate gene for the UBX family gene and could related with pectoral muscle structure of Korean native chicken.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGMENTS
LITERATURE CITED
참고문헌 (16)
참고문헌 신청
1. [학술지(정기간행물)] - Albrecht M / 2004 / Structural and functional analysis of ataxin-2 and ataxin-3 / Eur J Biochem 271 (15) : 3155 ~ 3170
2. [학술지(정기간행물)] - Arnett C / 1998 / The sequence of Homo sapiens PAC clone RP5-968I16 / Genome Res 8 (11) : 1097 ~ 1108
3. [학술지(정기간행물)] - Boardman PE / 2004 / Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution / Nature 432 (7018) : 695 ~ 716
4. [학술지(정기간행물)] - Caldwell RB / 2005 / Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis / Genome Biol 6 (1) : R6 ~
5. [학술지(정기간행물)] - Caravatti M / 1982 / Regulation of muscle gene expression: The accumulation of messenger RNAs coding for muscle-specific proteins during myogenesis in a mouse cell line / J Mol Biol 160 : 59 ~
2. [학술지(정기간행물)] - Arnett C / 1998 / The sequence of Homo sapiens PAC clone RP5-968I16 / Genome Res 8 (11) : 1097 ~ 1108
3. [학술지(정기간행물)] - Boardman PE / 2004 / Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution / Nature 432 (7018) : 695 ~ 716
4. [학술지(정기간행물)] - Caldwell RB / 2005 / Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis / Genome Biol 6 (1) : R6 ~
5. [학술지(정기간행물)] - Caravatti M / 1982 / Regulation of muscle gene expression: The accumulation of messenger RNAs coding for muscle-specific proteins during myogenesis in a mouse cell line / J Mol Biol 160 : 59 ~
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UCI(KEPA) : I410-ECN-0101-2013-527-000765294