지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 255 - 258 (4page)
이용수
초록· 키워드
오류제보하기
Eimeria tenella and Eimeria maxima are important pathogens causing intracellular protozoa infections in laboratory avian animals and are known to affect experimental results obtained from contaminated animals. This study aimed to find a fast, sensitive, and efficient protocol for the molecular identification of E. tenella and E. maxima in experimental samples using chickens as laboratory avian animals. DNA was extracted from fecal samples collected from chickens and polymerase chain reaction (PCR) analysis was employed to detect E. tenella and E. maxima from the extracted DNA. The target nucleic acid fragments were specifically amplified by PCR. Feces secreting E. tenella and E. maxima were detected by a positive PCR reaction. In this study, we were able to successfully detect E. tenella and E. maxima using the molecular diagnostic method of PCR. As such, we recommended PCR for monitoring E. tenella and E. maxima in laboratory avian facilities.
목차
Acknowledgments
References
참고문헌 (28)
참고문헌 신청
Dalloul RA / 2006 / Poultry coccidiosis: recent advancements in control measures and vaccine development / Expert Rev Vaccines 5(1):143~163
Wilson PA / 1961 / Biochemistry of sporulation in oocysts of Eimeria acervulina / J Protozool 8(4):410~416
Long PL / 1976 / A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis / Folia Vet Lat 6(4):201~217
Long PL / 1982 / A guide for the diagnosis of coccidiosis in chickens / Research report 404:1~17
Long PL / 1984 / Problems in identification of species of Eimeria / J Protozool 31(4):535~541
Wilson PA / 1961 / Biochemistry of sporulation in oocysts of Eimeria acervulina / J Protozool 8(4):410~416
Long PL / 1976 / A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis / Folia Vet Lat 6(4):201~217
Long PL / 1982 / A guide for the diagnosis of coccidiosis in chickens / Research report 404:1~17
Long PL / 1984 / Problems in identification of species of Eimeria / J Protozool 31(4):535~541
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UCI(KEPA) : I410-ECN-0101-2013-510-000764501