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자료유형
학술저널
저자정보
Yoon Ju Cho (Seojeong College University) Young Ah Lee (Konkuk University) Jae Won Lee (Konkuk University) Jong In Kim (Konkuk University) Jin Soo Han (Konkuk University)
저널정보
한국실험동물학회 Laboratory Animal Research Laboratory Animal Research Vol.27 No.3
발행연도
2011.9
수록면
197 - 203 (7page)

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Tribromoethanol (2,2,2-tribromoethanol, TBE) is a popular injectable anesthetic agent used in mice in Korea. Our goal was to assess the risks associated with side effects (lesions) in the abdominal cavity, especially at high doses. To understand the underlying pathophysiological changes, we examined levels of cytokines through ELISA of abdominal lavage fluid and spleen collected from mice treated with low and high-dose TBE. ICR mice were anesthetized using one of the following protocols: a combination of TBE 200 mg/kg (1.25%) and xylazine 10 mg/kg; TBE 400 mg/kg (1.25%); and TBE 400 mg/kg (2.5%). Administration of high-dose TBE (400 mg/kg) increased the interleukin-1β and interleukin-6 levels in the peritoneal cavity over the short term (<1 day) compared with sham controls and low-dose TBE (200 mg/kg) groups. Cytokine expression in the low-dose TBE group was similar to the control group, whereas in the high-dose TBE group cytokine levels were higher in abdominal lavage fluid and spleen over the long term (10 days post-injection). We conclude that a combination of TBE 200 mg/kg (1.25%) and xylazine (10mg/kg) is a safe and effective anesthetic for use in animals.

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Materials and Methods
Results
Discussion
Acknowledgments
References

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