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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 109 - 116 (8page)
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이 논문의 연구 히스토리 (3)
2010
cDNA Isolation of Possible Neuronal-type Calcium-Activated Chloride Channel from Xenopus laevis
Ra Mi Lee
,
Rae Hyung Ryu
,
Myeong Joong Kim
외 1명
한국실험동물학회 학술발표대회 논문집
2010.08
학술대회자료
Gene profiling of Colon-type Calcium-Activated Chloride Channels from Xenopus laevis
Ra Mi Lee
,
Rae Hyung Ryu
,
Myeong Joong Kim
외 1명
한국실험동물학회 학술발표대회 논문집
2010.08
학술대회자료
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
To clone the first anion channel from Xenopus laevis (X. laevis), we isolated a calcium-activated chloride channel (CLCA)-like membrane protein 6 gene (CMP6) in X. laevis. As a first step in gene isolation, an expressed sequence tags database was screened to find the partial cDNA fragment. A putative partial cDNA sequence was obtained by comparison with rat CLCAs identified in our laboratory. First stranded cDNA was synthesized by reverse transcription polymerase-chain reaction (RT-PCR) using a specific primer designed for the target cDNA. Repeating the 5’ and 3’ rapid amplification of cDNA ends, fulllength cDNA was constructed from the cDNA pool. The full-length CMP6 cDNA completed via 5’- and 3’-RACE was 2,940 bp long and had an open reading frame (ORF) of 940 amino acids. The predicted 940 polypeptides have four major transmembrane domains and showed about 50% identity with that of rat brain CLCAs in our previously published data. Semi-quantification analysis revealed that CMP6 was most abundantly expressed in small intestine, colon and liver. However, all tissues except small intestine, colon and liver had undetectable levels. This result became more credible after we did real-time PCR quantification for the target gene. In view of all CLCA studies focused on human or murine channels, this finding suggests a hypothetical protein as an ion channel, an X. laevis CLCA.
목차
Materials and Methods
Results
Discussion
Acknowledgments
References
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UCI(KEPA) : I410-ECN-0101-2013-510-000557319