지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 125 - 131 (7page)
이용수
초록· 키워드
오류제보하기
Through the present study, we produced a monoclonal antibody against aflatoxin B1 (AFB1) using AFB1-carboxymethoxylamine BSA conjugates. One clone showing high binding ability was selected and it was applied to develop a direct competitive ELISA system. The epitope densities of AFB1-CMO against BSA and KLH were about 1 : 6 and 1 : 545, respectively. The monoclonal antibody (mAb) from cloned hybridoma cell was the IgG1 subclass with λ-type light chains. The IC??s of the monoclonal antibody developed for AFB1, AFB2, AFG1 and AFG2 were 4.36, 7.22, 6.61 and 29.41 ng/㎖, respectively, based on the AFB1-KLH coated ELISA system and 15.28, 26.62, 32.75 and 56.67 ng/㎖, respectively, based on the mAb coated ELISA. Cross-relativities of mAb to AFB1 for AFB2, AFG1 and AFG2 were 60.47, 65.97 and 14.83% in the AFB1-KLH coated ELISA, and 59.41, 46.66 and 26.97% in the mAb coated ELISA, respectively. Quantitative calculations for AFB1 from the AFB1-Ab ELISA and AFB1-Ag ELISA ranged from 0.25 to 25 ng/㎖ (R² ＞ 0.99) and from 1 to 100 ng/㎖ (R² ＞ 0.99), respectively. The intra- and interassay precision CVs were ＜ 10% in both ELISA assay, representing good reproducibility of developed assay. Recoveries ranged from 79.18 to 91.27%, CVs ranged from 3.21 to 7.97% after spiking AFB1 at concentrations ranging from 5 to 50 ng/ml and following by extraction with 70% methanol solution in the Ab-coated ELISA. In conclusion, we produced a group specific mAb against aflatoxins and developed two direct competitive ELISAs for the detection of AFB1 in feeds based on a monoclonal antibody developed.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULT AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (25)
참고문헌 신청
Bailey, E.A. / 1996 / Mutational properties of the primary aflatoxin B1-DNA adduct / Proc. Natl. Acad. Sci 93:1535~1539
Burkin, A.A. / 2002 / [Group-specific antibodies against zearalenone and its metabolitesand synthetic analogs] / Prikl. Biokhim. Mikrobiol 38:194~202
Burkin, M.A. / 2000 / [Comparativeimmunochemical characteristics of polyclonal and monoclonalantibodies to aflatoxin B1] / Prikl. Biokhim. Mikrobiol 36:575~581
Celik, I. / 2000 / Efficacy of polyvinylpolypyrrolidone in reducingthe immunotoxicity of aflatoxin in growing broilers / Br.Poult. Sci 41:430~439
Cervino, C. / 2008 / Com-parison of hybridoma screening methods for the efficient detectionof high-affinity hapten-specific monoclonal antibodies / J.Immunol. Methods 329:184~193
Burkin, A.A. / 2002 / [Group-specific antibodies against zearalenone and its metabolitesand synthetic analogs] / Prikl. Biokhim. Mikrobiol 38:194~202
Burkin, M.A. / 2000 / [Comparativeimmunochemical characteristics of polyclonal and monoclonalantibodies to aflatoxin B1] / Prikl. Biokhim. Mikrobiol 36:575~581
Celik, I. / 2000 / Efficacy of polyvinylpolypyrrolidone in reducingthe immunotoxicity of aflatoxin in growing broilers / Br.Poult. Sci 41:430~439
Cervino, C. / 2008 / Com-parison of hybridoma screening methods for the efficient detectionof high-affinity hapten-specific monoclonal antibodies / J.Immunol. Methods 329:184~193
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UCI(KEPA) : I410-ECN-0101-2013-513-000636850