지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.3
- 수록면
- 65 - 70 (6page)
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초록· 키워드
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Development of mouse fetus brains can be defined morphologically and functionally by three developmental stages, embryo day (ED) 16, postnatal stage one week and eight weeks. These defined stages of brain development may be closely associated with differential gene expression rates due to limited cellular resources such as energy, space, and free water. Complex patterns of expressed genes and proteins during brain development suggests the changes in relative concentrations of proteins rather than the increase in numbers of new gene products. This study was designed to evaluate early protein expression pattern in mouse fetus brain. The mouse brain proteome of fetus at ED 15.5, and 19.5 was obtained using 2-dimensional gel electrophoresis (DE). Analysis of the 2-DE gels in pH 3-10 range revealed the presence of 15 differentially expressed spots, of which 11 spots were identified to be known proteins following MALDI-TOF analysis; 3 spots were up-regulated and 8 spots were down-regulated in the mouse fetus brain at ED 15.5. UP-regulated proteins were identified as MCG18238, isoform M2 of pyruvate kinase isozymes M1/M2, isoform 2 of heterogeneous nuclear ribonucleoprotein K, heterogeneous nuclear ribonucleoprotein H2, creatine kinase B-type, 40S ribosomal protein SA and hemoglobin subunit beta-H1. Down-regulated proteins were putative uncharacterized protein, lactoylglutathione lyase and secreted acidic cysteine rich glycoprotein. Our results revealed composite profiles of mouse fetus brain proteins related to mouse fetus development by 2-DE analysis implying possible roles of these proteins in neural differentiation.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 결론
감사의 글
참고문헌
참고문헌 (14)
참고문헌 신청
1. [학술지(정기간행물)] - Bradshaw AD / 2003 / SPARC-null mice exhibit increased adiposity without significant differences in overall body weight / Proc. Natl. Acad. Sci 100 : 6045 ~ 6050
2. [학술지(정기간행물)] - Görg A / 2000 / The current state of two-dimensional electrophoresis with immobilized pH gradients / Electrophoresis 21 : 1037 ~ 1053
3. [학술지(정기간행물)] - Gotz, M / 2005 / The cell biology of neurogenesis / Nat. Rev. Mol. Cell Biol 6 : 777 ~ 788
4. [학술지(정기간행물)] - Greenberg DA / 2006 / Neurodegeneration and neurogenesis: Focus on Alzheimer’s disease / Curr. Alzheimer Res 3 : 25 ~ 28
5. [학술지(정기간행물)] - Guillemot F / 2007 / Cell fate specification in the mammalian telencephalon / Prog. Neurobiol 134 : 3771 ~ 3780
2. [학술지(정기간행물)] - Görg A / 2000 / The current state of two-dimensional electrophoresis with immobilized pH gradients / Electrophoresis 21 : 1037 ~ 1053
3. [학술지(정기간행물)] - Gotz, M / 2005 / The cell biology of neurogenesis / Nat. Rev. Mol. Cell Biol 6 : 777 ~ 788
4. [학술지(정기간행물)] - Greenberg DA / 2006 / Neurodegeneration and neurogenesis: Focus on Alzheimer’s disease / Curr. Alzheimer Res 3 : 25 ~ 28
5. [학술지(정기간행물)] - Guillemot F / 2007 / Cell fate specification in the mammalian telencephalon / Prog. Neurobiol 134 : 3771 ~ 3780
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UCI(KEPA) : I410-ECN-0101-2012-480-004474158