지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.3
- 수록면
- 52 - 59 (8page)
이용수
초록· 키워드
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Recently, new ginseng cultivars having superior agricultural traits have been developed in Korea. For newly developed plant cultivars, the identifi cation of distinctiveness is very important factors not only in plant cultivar management but also in breeding programs. Thus, eighty-fi ve inter simple sequence repeat (ISSR) primers were applied to detect polymorphisms among six major Korean ginseng cultivars and two foreign ginsengs. A total of 197 polymorphic bands with an average 5.8 polymorphic bands and 2.9 banding patterns per assay unit across six Korean ginseng cultivars and foreign ginsengs from 236 amplifi ed ISSR loci with an average 6.9 loci per assay unit were generated by 34 out of 85 ISSR primers. Three species of Panax ginseng including the Korean ginseng cultivars, P. quinquefolius, and P. notoginseng, could be readily discriminated using most tested primers. UBC-821, UBC-868, and UBC-878 generated polymorphic bands among the six Korean ginseng cultivars, and could distinguish them from foreign ginsengs. Sequence characterized amplifi ed region (SCAR) marker system was introduced in order to increase the reproducibility of the polymorphism. One SCAR marker, PgI821C650, was successfully converted from the randomly amplifi ed polymorphism by UBC-821. It showed the expected dominant polymorphism among ginseng samples. In addition, the specifi c polymorphism for Sunwon was generated by treating Taq I restriction enzyme to polymerase chain reaction products of PgI821C650. These results will serve as useful DNA markers for identifi cation of Korean ginseng, especially Sunwon cultivar, seed management, and molecular breeding program supplemented with marker-assisted selection.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - Wen J / 1996 / Phylogeny and biogeography of Panax L. (the ginseng genus, araliaceae): inferences from ITS sequences of nuclear ribosomal DNA / Mol Phylogenet Evol 6 : 167 ~ 177
2. [학술지(정기간행물)] - Liu J / 1995 / Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly / Mech Ageing Dev 83 : 43 ~ 53
3. [학술지(정기간행물)] - Shin HR / 2000 / The cancerpreventive potential of Panax ginseng: a review of human and experimental evidence / Cancer Causes Control 11 : 565 ~ 576
4. [학술지(정기간행물)] - Yun TK / 2001 / Anticarcinogenic effect of Panax ginseng C. A. Meyer and identifi - cation of active compounds / J Korean Med Sci 16 : S6 ~ S18
5. [학술지(정기간행물)] - Dey L / 2003 / Anti-hyperglycemic effects of ginseng: comparison between root and berry / Phytomedicine 10 : 600 ~ 605
2. [학술지(정기간행물)] - Liu J / 1995 / Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly / Mech Ageing Dev 83 : 43 ~ 53
3. [학술지(정기간행물)] - Shin HR / 2000 / The cancerpreventive potential of Panax ginseng: a review of human and experimental evidence / Cancer Causes Control 11 : 565 ~ 576
4. [학술지(정기간행물)] - Yun TK / 2001 / Anticarcinogenic effect of Panax ginseng C. A. Meyer and identifi - cation of active compounds / J Korean Med Sci 16 : S6 ~ S18
5. [학술지(정기간행물)] - Dey L / 2003 / Anti-hyperglycemic effects of ginseng: comparison between root and berry / Phytomedicine 10 : 600 ~ 605
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UCI(KEPA) : I410-ECN-0101-2012-524-004215825