지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.3
- 수록면
- 31 - 38 (8page)
이용수
초록· 키워드
오류제보하기
In order to distinguish the cultivation area of Panax ginseng, principal component analysis (PCA) using quantitative and qualitative data acquired from HPLC was carried out. A new HPLC method coupled with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous quantification of ten major ginsenosides, namely Rh₁, Rg₂, Rg₃, Rg₁, Rf, Re, Rd, Rb₂, Rc, and Rb₁ in the root of P. ginseng C. A. Meyer. Simultaneous separations of these ten ginsenosides were achieved on a carbohydrate analytical column. The mobile phase consisted of acetonitrile-water-isopropanol, and acetonitrile-waterisopropanol using a gradient elution. Distinct differences in qualitative and quantitative characteristics for ginsenosides were found between the ginseng roots produced in two different Korean cultivation areas, Ganghwa and Punggi. The ginsenoside profiles obtained via HPLC analysis were subjected to PCA. PCA score plots using two principal components (PCs) showed good separation for the ginseng roots cultivated in Ganghwa and Punggi. PC1 infl uenced the separation, capturing 43.6% of the variance, while PC2 affected differentiation, explaining 18.0% of the variance. The highest contribution components were ginsenoside Rg₃ for PC1 and ginsenoside Rf for PC2. Particularly, the PCA score plot for the small ginseng roots of six-year old, each of which was light than 147 g fresh weight, showed more distinct discrimination. PC1 infl uenced the separation between different sample sets, capturing 51.8% of the variance, while PC2 affected differentiation, also explaining 28.0% of the variance. The highest contribution component was ginsenoside Rf for PC1 and ginsenoside Rg2 for PC2. In conclusion, the HPLC-ELSD method using a carbohydrate column allowed for the simultaneous quantifi cation of ten major ginsenosides, and PCA analysis of the ginsenoside peaks shown on the HPLC chromatogram would be a very acceptable strategy for discrimination of the cultivation area of ginseng roots.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (21)
참고문헌 신청
1. [학술지(정기간행물)] - Akerele O / 1992 / WHO guideline for assessment of herbal medicines / Fitoterapia 63 : 99 ~ 104
2. [학술지(정기간행물)] - Wang M / 2005 / Metabolomics in the context of systems biology: bridging traditional Chinese medicine and molecular pharmacology / Phytother Res 19 : 173 ~ 182
3. [학술지(정기간행물)] - Cicero AF / 2003 / Panax notoginseng(Burk.) effects on fi brinogen and lipid plasma level in rats fed on a high-fat diet / Phytother Res 17 : 174 ~ 178
4. [학술지(정기간행물)] - Sun H / 2006 / Structure and biological activity of protopanaxatriol-type saponins from the roots of Panax notoginseng / Int Immunopharmacol 6 : 14 ~ 25
5. [학술지(정기간행물)] - Hye-Min Lee / 2010 / Effect of Ginsenoside Rg3 and Rh2 on Glucose Uptake in Insulin-resistant Muscle Cells / Journal of the Korean Society for Applied Biological Chemistry 53 (1) : 106 ~ 109
2. [학술지(정기간행물)] - Wang M / 2005 / Metabolomics in the context of systems biology: bridging traditional Chinese medicine and molecular pharmacology / Phytother Res 19 : 173 ~ 182
3. [학술지(정기간행물)] - Cicero AF / 2003 / Panax notoginseng(Burk.) effects on fi brinogen and lipid plasma level in rats fed on a high-fat diet / Phytother Res 17 : 174 ~ 178
4. [학술지(정기간행물)] - Sun H / 2006 / Structure and biological activity of protopanaxatriol-type saponins from the roots of Panax notoginseng / Int Immunopharmacol 6 : 14 ~ 25
5. [학술지(정기간행물)] - Hye-Min Lee / 2010 / Effect of Ginsenoside Rg3 and Rh2 on Glucose Uptake in Insulin-resistant Muscle Cells / Journal of the Korean Society for Applied Biological Chemistry 53 (1) : 106 ~ 109
이 논문의 저자 정보
이 논문과 함께 이용한 논문
New Method for Simultaneous Quantifi cation of 12 Ginsenosides in Red Ginseng Powder and Extract : In-house Method Validation
Gyo In
,
Nam-Geun Ahn
,
Bong-Seok Bae
외 3명
Journal of Ginseng Research
2012
.04
HPLC를 이용한 고려인삼 중 진세노사이드 Rb₁, Rf 및 Rg₁의 신속분석 방법 개발
홍희도
,
최상윤
,
김영찬
외 2명
Journal of Ginseng Research
2009
.03
A new validated analytical method for the quality control of red ginseng products
Il-Woung Kim
,
Kyu-Min Cha
,
Jae Joon Wee
외 2명
Journal of Ginseng Research
2013
.10
Distinction of Internal Tissue of Raw Ginseng Root Using a Computed Tomography Scanner
In-Chan Jung
,
In Soo Jeong
,
Cheon-Suk Kim
Journal of Ginseng Research
2012
.10
Comparison of Ginsenoside and Phenolic Ingredient Contents in Hydroponically-cultivated Ginseng Leaves, Fruits, and Roots
Sang Yoon Choi
,
Chang-Won Cho
,
Yeonmi Lee
외 3명
Journal of Ginseng Research
2012
.10
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2012-524-004215794