In this paper, we propose an antenna module with high isolation between Tx and Rx in RFID reader module. The proposed module consists of a quadrafilar antenna and a directional coupler with a switchable dummy load to improve the directivity. The proposed module achieves the isolation higher than 20 ㏈ between Tx and Rx. To show the validity of the proposed scheme, we have performed the measurement of tagging range and multi-tagging ability. The experiment results show that the detecting range and multi-tagging ability are enhanced by 81% and 200%, respectively.