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논문 기본 정보

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학술저널
저자정보
Yeon Kyung Lee (부산대학교) Ji Eun Kim (부산대학교) So Hee Nam (부산대학교) Sun il Choi (부산대학교) Eon Pil Lee (부산대학교) Hae Wook Choi (부산대학교) Hong Sung Kim (부산대학교) Jae Ho Lee (부산대학교) Young Jin Jung (부산대학교) Byeong Cheol Kang (서울대학병원) Jung Sik Cho (호서대학교) Dae Youn Hwang (부산대학교)
저널정보
한국실험동물학회 Laboratory Animal Research Laboratory Animal Research Vol.26 No.3
발행연도
2010.9
수록면
233 - 239 (7page)

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Guided bone regeneration (GBR) is a technique that a barrier membrane is placed over the bone defect to prevent the cell growth from the connective tissue and epithelium. In this study, in order to determinewhether GBR technique could induce stress in rats, the standardized bone defect in rat calvaria was covered with apatitte membrane. Bone and brain tissues were collected from rats at 3 days, 2, 4, and 16 weeks post-operation, and then alteration of the new bone formation at the defects and stress-related factors were detected with histological examination and Western blot, respectively. From 4 to 16 weeks after the operation, the apatitte membrane was attached to the region of regenerated bone and encapsulated with a thick fibrous layer. Furthermore, the concentration of cortisol, a good indicator of stress, significantly increased 3 days post-operation. However, the increase at 3 days was returned to the basal level in 2 weeks. In Western blot analysis, the highest phosphorylation level of extracellular signalregulated kinase (ERK) was observed 3 day post-operation, while those of the c-jun N-terminal kinase (JNK) and p38 were detected 4 weeks post-operation. Taken together, the results suggest that GBR technique may induce the serious stress on the brain tissue via the induction of ERK phosphorylation during 2 weeks, and that the stress responses restored in 4 week via JNK and p38 signaling pathway.

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