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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.51 No.3
- 발행연도
- 2010.6
- 수록면
- 222 - 225 (4page)
이용수
초록· 키워드
오류제보하기
In vitro zoysia grass regeneration has several difficulties. We report here a simple and reproducible protocol by using seed explants. We selected the yellow colored calli for plant regeneration, as other types of calli were found to be poor in their capacity for callus multiplication and/or shoot induction. However, both previous reports and the present study encountered difficulties in plant regeneration with the yellow type calli during their growth and shoot multiplication. Some of the major difficulties included the formation of different types of calli from a single explant that appeared to be of a regenerable type of calli (yellow compact, yellowish white compact and white friable), abnormal growth induction from the callus (hairy root formation, browning, and formation white compact structures resembling somatic embryos), greening of callus without further shoot formation, micro-shoot formation without further growth and conversion of micro-shoots into callus. To circumvent these difficulties, we established an improved protocol for zoysia regeneration. Results showed that 2iP (1.0 ㎎ㆍℓ?¹) and GA3 (1.0 ㎎ㆍℓ?¹) not only enhanced shoot induction percentage, but also increased the number of shoots from the callus cultures when compared with BAP treatments. During shoot initiation, the difficulties mentioned above were substantially alleviated. Both shoot induction and simultaneous shoot elongation were satisfactorily achieved on the same medium. The elongated shoots were rooted on half MS media. The simple but reproducible regeneration protocol established in this study may facilitate the work on transformation and genetic improvements for Zoysia japonica Steud. and other closely related grass species.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited
참고문헌 (16)
참고문헌 신청
1. [학술지(정기간행물)] - Bae, C.H. / 2001 / Efficient plant regeneration using mature seedderived callus in zoysiagrass (Zoysia japonica Steud.) / Kor. J. Plant Tissue Culture 28 : 61 ~ 67
2. [학술지(정기간행물)] - Cardona, C.A. / 1997 / Callus induction and high efficiency plant regeneration via somatic embryogenesis in paspalum / Crop Sci 37 : 1297 ~ 1302
3. [학술지(정기간행물)] - Chaudhury, A. / 2000 / Somatic embryogenesis and plant regeneration of turf type bermudagrass : Effect of 6-benzyladenine in callus induction medium / Plant Cell Tiss. Org. Cult 60 : 113 ~ 120
4. [학술지(정기간행물)] - Dhandapani, M. / 2008 / Regeneration of zoysia grass (Zoysia matrella L. Merr.) cv. Konhee from young inflorescences and stem nodes / In Vitro Cell. Dev. Biol. –Plant 44 : 8 ~ 13
5. [단행본] - Duble, R.L. / 1996 / Turfgrasses: their management and use in the southern zone / Texas A&M University Press
2. [학술지(정기간행물)] - Cardona, C.A. / 1997 / Callus induction and high efficiency plant regeneration via somatic embryogenesis in paspalum / Crop Sci 37 : 1297 ~ 1302
3. [학술지(정기간행물)] - Chaudhury, A. / 2000 / Somatic embryogenesis and plant regeneration of turf type bermudagrass : Effect of 6-benzyladenine in callus induction medium / Plant Cell Tiss. Org. Cult 60 : 113 ~ 120
4. [학술지(정기간행물)] - Dhandapani, M. / 2008 / Regeneration of zoysia grass (Zoysia matrella L. Merr.) cv. Konhee from young inflorescences and stem nodes / In Vitro Cell. Dev. Biol. –Plant 44 : 8 ~ 13
5. [단행본] - Duble, R.L. / 1996 / Turfgrasses: their management and use in the southern zone / Texas A&M University Press
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