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논문 기본 정보

자료유형
학술저널
저자정보
Chang Hee Lee (서울대학교) Ki Sun Kim (서울대학교)
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.51 No.2
발행연도
2010.4
수록면
115 - 122 (8page)

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An efficient and reproducible micropropagation method for Dendranthema zawadskii complex, which have troubleshooting such as heterogeneous seeds, limitation of shoot tip cuttings, and infected rhizomes in conventional propagation, was developed through direct shoot organogenesis. In this experiment, eight taxa of Dendranthema zawadskii complex native to Korea were used as plant materials such as D. zawadskii ssp. acutilobum (ACU), D. zawadskii ssp. acutilobum var. alpinum (ALP), D. zawadskii ssp. acutilobum var. tenuisectum (TEN), D. zawadskii ssp. coreanum (COR), D. zawadskii ssp. latilobum (LAT), D. zawadskii ssp. lucidum (LUC), D. zawadskii ssp. naktongense (NAK), and D. zawadskii ssp. yezoense (YEZ). High-frequency direct shoot organogenesis was induced on MS medium supplemented with 0.5-5.0 ㎎?L<SUP>-1</SUP> 6-benzylaminopurine (BAP) and 0.01 ㎎?L<SUP>-1</SUP> α-naphthaleneacetic acid (NAA). Optimum BAP concentration for multiplication varied among taxa. BAP at 0.5 ㎎?L<SUP>-1</SUP> was optimal to produce 65-115 adventitious shoots from single explant of the four taxa (TEN, COR, LAT, and YEZ) after 28 d of in vitro culture, whereas BAP at 2.0 ㎎?L<SUP>-1</SUP> was optimal to produce 10-60 adventitious shoots from single explant of the three taxa (ACU, LUC, and NAK) after 28 d of in vitro culture. Shoot-forming capacity was influenced by the number of adventitious shoots attached to propagule used as starting material for multiple shoot proliferation and synchronized effect was observed. In ACU and LUC, higher multiplication efficiency (115~120%) was found when propagules with two shoots were used than with only one shoot (100%). Roots were successfully formed regardless of the presence of auxin, while indole-3-butyric acid (IBA) at 0.1-0.5 ㎎?L<SUP>-1</SUP> enhanced root development of COR.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2010-525-003282792