지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.3
- 수록면
- 91 - 94 (4page)
이용수
초록· 키워드
오류제보하기
Damaged DNA binding (DDB) protein is an important gene in the repair of damaged DNA. DDB is a heterodimer (DDB1 and DDB2) protein, murine DDB2 has 10 exons about 1.5kb in size (Genbank Accession No. AY027937). Here we identified five DDB2 variants (M1-M5) from various mouse tissues that are generated by alternative splicing. We used reverse transcription-PCR (RT-PCR) to identify splicing variants and isolated PCR products using an agarose-gel PCR purification kit. All isolated PCR products were cloned and the structure of splicing variants was confirmed by sequencing. The first splicing variant M1 was generated by omission of exon 4. The second splicing variant M2, by omission of exons 4-5. The third variants M3 was generated by omission from the middle of exon 1 to exon 6 and was expressed in the heart. Fourth variants M4 was generated by omission of exon 2 and exons 4-7. M5, the last splicing variant was generated by omission of exons 4-7. M4 and M5 were expressed in the spleen. Analysis of tissue distribution by RT-PCR indicates that M1 is most highly expressed in the mouse brain. These results indicated that murine DDB2 has five splicing variants and splicing variants expression patterns were different depending on mouse tissue. Further functional studies of each splicing variants will provide more information about the molecular mechanism of DDB2 function and DDB2 gene expression regulation.
목차
Materials and Methods
Results and Discussion
Acknowledgments
References
참고문헌 (18)
참고문헌 신청
1. [학술지(정기간행물)] - Aboussekhra, A. / 1995 / Mammalian DNA nucleotide excision repair reconstituted with purified protein components / Cell 80 (6) : 859 ~ 868
2. [학술지(정기간행물)] - Black, D.L. / 2003 / Mechanisms of alternative pre-messenger RNA splicing / Annu. Rev. Biochem. 72 : 291 ~ 336
3. [학술지(정기간행물)] - Bohr, V.A. / 1985 / DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall / Cell 40 (2) : 359 ~ 369
4. [학술지(정기간행물)] - David, C.J. / 2008 / The search for alternative splicing regulators: new approaches offer a path to a splicing code / Genes Dev. 22 (3) : 279 ~ 285
5. [학술지(정기간행물)] - Fedor, M.J. / 2008 / Alternative splicing minireview series: combinatorial control facilitates splicing regulation of gene expression and enhances genome diversity / J. Biol. Chem. 283 (3) : 1209 ~ 1210
2. [학술지(정기간행물)] - Black, D.L. / 2003 / Mechanisms of alternative pre-messenger RNA splicing / Annu. Rev. Biochem. 72 : 291 ~ 336
3. [학술지(정기간행물)] - Bohr, V.A. / 1985 / DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall / Cell 40 (2) : 359 ~ 369
4. [학술지(정기간행물)] - David, C.J. / 2008 / The search for alternative splicing regulators: new approaches offer a path to a splicing code / Genes Dev. 22 (3) : 279 ~ 285
5. [학술지(정기간행물)] - Fedor, M.J. / 2008 / Alternative splicing minireview series: combinatorial control facilitates splicing regulation of gene expression and enhances genome diversity / J. Biol. Chem. 283 (3) : 1209 ~ 1210
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UCI(KEPA) : I410-ECN-0101-2010-510-003342861