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자료유형
학술저널
저자정보
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한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.51 No.1
발행연도
2010.2
수록면
51 - 60 (10page)

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Walnut, (Juglans regia), is one of the most important nutritive nut crops. Iran is considered as one of the centers for diversity and cultivation of walnut in middle-eastern part of the world. In this study, some morphological traits in combination with polymorphic RAPD primers were applied to evaluate genetic potential of 35 (31 Iranian and four foreign) walnut genotypes. From 14 RAPD primers, 180 DNA bands were amplified among which 174 were highly polymorphic. The lowest and the highest pair-wise similarity coefficients between examined genotypes were 0.27 and 0.89, respectively. Estimated resolving power index (Rp) were from 2.17 (opAD16) to 8.40 (opAA19) and total resolving power (Rp) 70.61 in all 14 RAPD loci. Cluster analysis based on Jaccard's similarity coefficients and UPGMA method divided the genotypes into five groups at similarity of 0.50, which the main group consisted of 31 genotypes including 4 foreign cultivars. Genetic variation among locations took 9.48% of the total genetic variation detected with Analysis of molecular variances (AMOVA) (p<0.001). Most Iranian genotypes grouped closely with foreign cultivars and showed high similarities indicating their close genetic relatedness. RAPD markers showed to be an efficient technique for studying genetic diversity of walnut genotypes. These genotypes with different genetic bases and suitable horticultural traits seem to be good candidates applicable for direct use as vegetatively propagated material or as parents in walnut improvement programs.

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Abstract
Introduction
Materials and Methods
Result and Discussion
Discussion
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2010-525-002268270