지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.12
- 수록면
- 322 - 327 (6page)
이용수
초록· 키워드
오류제보하기
A total of 23 isolates of Botrytis cinerea causing the grey mold were collected from infected ginseng in several fields of Korea. The sensitivity to carbendazim and the mixture of carbendazim plus diethofencarb was determined through a mycelial inhibition test on PDA amended with or without fungicides. B. cinerea isolates were classified as 3 phenotypes, which were the first phenotype resistant to both of carbendazim and the mixture (Car<SUP>R</SUP>Mix<SUP>R</SUP>), the second one resistant to carbendazim and sensitive to the mixture (Car<SUP>R</SUP>Mix<SUP>S</SUP>), and the last one sensitive to both of them (Car<SUP>S</SUP>Mix<SUP>S</SUP>). Carbendazim resistance correlated with a single mutation in β- tubulin gene of B. cinerea amplified with primer pair tubkjhL and tubkjhR causing a change of glutamate to alanine at amino acid position 198. Furthermore, the substitution of valine for glutamate led the resistance to carbendazim and the mixture at the same position of amino acid. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis using the restriction endonuclease, Tsp45I and BstUI allowed differentiation of the PCR fragment of β-tubulin gene of Car<SUP>S</SUP>Mix<SUP>S</SUP> isolates from that of Car<SUP>R</SUP>Mix<SUP>R</SUP> and Car<SUP>R</SUP>Mix<SUP>S</SUP> isolates. This method will aid in a fast detection of resistance of carbendazim and the mixture of carbendazim plus diethofencarb in B. cinerea in ginseng field.
목차
Materials and Methods
Results
Discussion
Acknowledgements
References
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UCI(KEPA) : I410-ECN-0101-2010-481-001620246