지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.2
- 발행연도
- 2009.4
- 수록면
- 148 - 153 (6page)
이용수
초록· 키워드
오류제보하기
Micropropagation of walnut has been partially limited to the lack of an appropriate culture medium. We hypothesized that the minerals in proportions similar to those found in the walnut seeds could provide an optimum medium for shoot micropropagation. The mineral composition of five walnut seeds as ‘Serr’, ‘Pedro’, ‘Lara’, ‘Hartley’ and ‘Rond e Montignac’ were analyzed by Inductive Couple Plasma-Omission Spectroscopy (ICP-OES). In addition, to study the effect of Cu and myo-inositol on shoot length and rooting rate of explants, these factors were examined. Concentrations of mineral compositions of walnut seeds were several times of those in DKW (Driver and Kuniyuki Walnut medium). Based on minimum concentration of seeds, two new media (as ×1.5 and ×2 DKW) were formulated. Growth of explants of ’Hartley‘, ‘Sunland’ and ‘Vina’ cultivars on two new media was compared to those cultured on DKW medium. The explants cultured on ×2 DKW and ×1.5 DKW formed shoots having green-colored leaves but produced callus similar to those cultured on DKW medium. Better growth was observed on ×1.5 DKW medium. Potential multiplication rate was different among the cultivars and ‘Vina’ had the maximum stem length and number of auxiliary buds on ×1.5 DKW. The results of the second experiment indicated that number of auxiliary buds per shoot and stem length were enhanced in higher levels of Cu and myo-inositol. In vitro rooting percentage for ‘Sunland’, ‘Hartley’ and ‘Vina’ were 50, 31 and 16% in the DKW medium, respectively but decreased to 5-17% in two modified media (×1.5 and ×2 DKW). A better rooting (up to 70%) was obtained when shoots were placed in higher levels of Cu and myo-inositol (CM2). The results suggested that some of the walnut cultivars need to grow on higher mineral concentrations of DKW medium. In addition, the higher levels of Cu and myo-inositol would be affective on growth rate and rooting percentage of walnut explants.
목차
Abstract
Introduction
Material and Methods
Results and Discussion
Acknowledgments
Literature Cited
참고문헌 (34)
참고문헌 신청
1. [단행본] - Bandurski,R.S / 1980 / Homeostatic control of concentrations of indole -3-acetic acid. Plant Growth Substances / Springer : 37 ~ 49
2. [학술지(정기간행물)] - Birmeta, G / 2004 / Efficient micropropagation of Ensete ventricosum applying meristem wounding: A three step protocol / Plant Cell Rep 23 : 277 ~ 283
3. [학술지(정기간행물)] - Chenevard, D / 1995 / The effect of sucrose on the development of hybrid walnut microcuttings (Juglans nigra × Juglans regia). Consequences on their survival during acclimatization / Ann. Sci. For 52 : 147 ~ 156
4. [학술지(정기간행물)] - Cho, M.J / 1998 / Transformation of recalcitrant barley cultivars through improvement of regenerative ability and decreased albinism / Plant Sci 138 : 229 ~ 244
5. [학술지(정기간행물)] - Cohen, J.D / 1982 / Chemistry and physiology of the bound auxins / Ann. Rev. Plant Physiol 33 : 403 ~ 430
2. [학술지(정기간행물)] - Birmeta, G / 2004 / Efficient micropropagation of Ensete ventricosum applying meristem wounding: A three step protocol / Plant Cell Rep 23 : 277 ~ 283
3. [학술지(정기간행물)] - Chenevard, D / 1995 / The effect of sucrose on the development of hybrid walnut microcuttings (Juglans nigra × Juglans regia). Consequences on their survival during acclimatization / Ann. Sci. For 52 : 147 ~ 156
4. [학술지(정기간행물)] - Cho, M.J / 1998 / Transformation of recalcitrant barley cultivars through improvement of regenerative ability and decreased albinism / Plant Sci 138 : 229 ~ 244
5. [학술지(정기간행물)] - Cohen, J.D / 1982 / Chemistry and physiology of the bound auxins / Ann. Rev. Plant Physiol 33 : 403 ~ 430
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