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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.2
- 발행연도
- 2009.4
- 수록면
- 109 - 115 (7page)
이용수
초록· 키워드
오류제보하기
A nutrient-circulated micropropagation system using a scaled-up vessel was developed for photoautotrophic micropropagation of potato plantlets. To verify usefulness of this system, potato (Solanum tuberosum L. cv. Dejima) plantlets were micropropagated under three different systems: nutrient-circulated photoautotrophic micropropagation system using a scaled-up vessel (PAS), photoautotrophic micropropagation using a magenta vessel (PAM), and photomixotrophic micropropagation using a magenta vessel (PMM). The growth of potato plantlets under PAS was significantly improved compared to those under PAM and PMM due to adequate CO₂ supply by forced ventilation and better root-zone environment conditions through circulated nutrient solution. This result on in vitro growth directly affected to survival rate and ex vitro growth. In addition, to determine proper ventilation rate in PAS, potato plantlets were grown under scaled-up vessels with different numbers of air exchanges (4, 7, 10, and 13 h<SUP>-1</SUP>). The number of air exchanges had an impact on relative humidity rather than CO₂ concentration in the vessels. Low relative humidity (70-80%) by high numbers of air exchanges (10, and 13 h<SUP>-1</SUP>) induced well-developed guard cells and wax deposition on the leaves of potato plantlets. Furthermore, high numbers of air exchanges (10, and 13 h<SUP>-1</SUP>) promoted in vitro growth and subsequently ex vitro growth after transplanting. Thus, it is concluded that PAS was suitable for the mass production of potato plantlets; also, to obtain vigorous growth of potato plantlets cultured in PAS, the number of air exchanges should be set at 10 h<SUP>-1</SUP> or over by forced ventilation.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
Literature Cited
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