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학술저널
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충남대학교 농업과학연구소 Korean Journal of Agricultural Science 農業科學硏究 第35卷 第2號
발행연도
2008.12
수록면
167 - 176 (10page)

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Supplementation of serum and estrogen in in vitro maturation(IVM) medium was shown to improve embryo development and quality in several species. This study investigates the effect of ovarian estrus stage on canine oocyte quality and supplementation of medium with canine serum or estrogen on IVM of canine oocytes. As results, in experimental 1, IVM oocytes collected from follicular stage ovaries to MII stages(10.2±1.5%) was higher (p<0.05) with 10% canine estrus stage serum than control(1.3±1.6%), anoestrus stage serum(4.0±1.6%), luteal stage serum (2.7±1.7%) and 10% FBS(1.3±1.6). In experimental 2, 10% canine estrus stage serum supplementation has highest maturation rate to MII stages(10.0±1.8%) and there were significant differences(P<0.05) with another treatment in follicular stages group. In order to investigate the synergic effect of estrous serum and estrogen supplementation, different estrous stage groups of oocytes were cultured with 2 ug/ml estrogen plus various concentrations of different reproductive stage serum and FBS(experimental 3). As results, the rate of maturation to metaphase Ⅱ(MII) stage was significantly higher(p<0.05) in oocytes from the follicular stage supplemented with estrogen and 10% canine estrus stage serum(11.5%) compared to the other groups(6.0?8.8%). The present study was demonstrated that canine serum and the estrus cycle of the bitch affect the meiotic competence of oocytes. Hormonal influences within the follicle may be one of the factors responsible for the greater proportion of maturation of oocyte to MII from bitches at the follicular phase.

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ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIAL AND METHODS
Ⅲ. RESULTS
Ⅳ. DISCUSSION
References

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