지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The tetrachloroethylene (PCE) dehalogenase of Clostridium bifermentans DPH-1 (a halorespiring organism) was purified, cloned, and sequenced. This enzyme is a homodimer with a molecular mass of ca. 70 kDa and exhibits dehalogenation of dichloroethylene isomers along with PCE and trichloroethylene (TCE). Broad range of substrate specificity for chlorinated aliphatic compounds (PCE, TCE, cis-1,2-dichloroethylene, trans-1,2-dichloroethylene, 1,1-dichloroethylene. 1,2-dichloropropene, and 1,1,2-trichloroethane) for this enzyme was also observed. A mixture of propyl iodide and titanium citrate caused a light-reversible inhibition of enzymatic activity suggesting the involvement of a corrinoid cofactor. A partial sequence (81 bp) of the encoding gene for PCE dehalogenase was amplified and sequenced with degenerateprimers designed from the N-terrninal sequence (27 amino acid residues). Southern analysis of C. biiermentsns genomic DNA using the polymerase chain reaction product as a probe revealed restriction fragment bands. A 5.0 kb ClaI fragment, harboring the relevant gene (designated pceC) was cloned (pDEHAL5) and the complete nucleotide sequence of pceC was determined. The gene showed homology mainly with microbial membrane proteins and no homology with any known dehalogenase, suggesting a distinct PCE dehalogenase. So, C. bitermentsns could play some important role in the initial breakdown of PCE and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances.
목차
Abstract
Ⅰ. INTRODUCTION
Ⅱ. METHODS
Ⅲ. RESULTS
Ⅳ. CONCLUSION AND PERSPECTIVES
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Dechlorination of High Concentrations of Tetrachloroethylene Using a Fixed-bed Reactor
한국환경보건학회지
2010 .01
-
어린이용품중의 TCE&PCE 시험방법 정립 연구
환경독성보건학회 심포지엄 및 학술대회
2013 .05
-
DNA Single Strand Breaks of Perchloroethylene and Its Bio-degradation Products by Single Cell Gel Electrophoresis Assay in Mammalian Cell System
Molecular & cellular toxicology
2005 .01
-
Change in bacterial composition in fecal of weaning piglets supplemented with Phellodendron Cortex extract
예방수의학회지
2020 .01
-
캠프캐럴 주변지역 주민의 지하수 음용과 TCE, PCE 농도와의 관련성 연구
환경독성보건학회 심포지엄 및 학술대회
2013 .10
-
캠프 캐럴 인근 주민의 혈중 및 요중 휘발성 유기화합물 평가
한국산업보건학회지
2016 .01
-
Perchlotoethylene을 사용하는 세탁소 작업자들의 직업성 폭로와 건강에 관한 연구
한국산업보건학회지
1994 .01
-
신규 퀴놀론 항균제 DW-116의 소핵시험
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
1996 .01
-
Synthesis, Characterization and in vitro Anti-Tumoral Evaluation of Erlotinib-PCEC Nanoparticles
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
A Case of Clostridium bifermentans Bacteremia in a Patient with Myelodysplastic Syndrome
Infection and Chemotherapy
2011 .01
-
Poria cocos ethanol extract and its active constituent, pachymic acid, modulate sleep architectures via activation of GABAA-ergic transmission in rats
Journal of Biomedical and Translational Research
2015 .01
-
Single strand breakage of PCE, DCE, TCE and VC by single cell gel eletrophoresis assay in mammalian cell system
환경독성보건학회 심포지엄 및 학술대회
2005 .05
-
Single strand breakges of PCE, DCE, TCE and VC by single cell gel electrophoresis assay in mammalian cell system
환경독성보건학회 심포지엄 및 학술대회
2005 .11
-
일개 석영 가공 공장의 TCE와 PCE노출 작업자의 생물학적 모니터링
대한직업환경의학회 학술대회 논문집
2017 .11
-
Hepatic enzyme expression in tetrachloroethylene-inhaled mouse
한국실험동물학회 학술발표대회 논문집
2019 .07
-
컴퓨터 이미지 분석법을 이용한 마우스 골수세포에서 소핵의 계수
Toxicological Research
2002 .12
-
Micronucleus Test of Picrorrhiza Rhizoma Aqueous Extract in Bone Marrow Cells of Male ICR Mice
Toxicological Research
2011 .06
-
A Study of 90-day Inhalation Toxicity of Tetrachloroethylene
환경독성보건학회 심포지엄 및 학술대회
2017 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-517-019433137