지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.12
- 수록면
- 675 - 680 (6page)
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This study was conducted to establish mating procedure, pregnancy diagnosis and Caesarean section in Cynomolgus monkeys. Six males, aged 6~7 years, weighing 4~7 ㎏, and 18 females showing regular menstrual cycle, aged 4.5~6 years, weighing 3~5.6 ㎏ were selected. Mating was conducted daily for three consecutive days among 11 to 15 days after the menstruation start. As to mating, we established
the natural mating method, which cohabited male and female monkeys in a cage and produced mated animals. As a result of three days’ natural mating, 15 out of 18 female animals (83%) succeeded in mating, and 8 out of the mated ones (53%) succeeded in pregnancy. Mating was confirmed through observing the mating behavior and checking sperm in vagina smear. Pregnancy diagnosis and observation of embryonic development were performed using ultrasonic diagnostic equipment. According to the results of the tests, the endometrial cavity was opened by around a third on Day 18 of pregnancy, and the egg yolk and heart rate of the endometrial cavity were observed from Day 25. The fetal form was observed to some degree on Day 35, and the completed form of fetus was observed on Day 45. Caesarean section was conducted on Day 50 of pregnancy. To anesthetize pregnant animals, we performed intramuscular injection of tranquilizer, which was 0.1 mg/kg of medetomidine. We also applied intramuscular injection of 10 mg/kg of ketamine as an anesthetic and maintained oxygen and 1-2% of isoflurane using a mask to maintain anesthesia during operation. The condition of the gravid uterus, the survival and the external examination of the fetus were observed. In conclusion, the natural mating method established in this study was found to be a useful tool for obtaining pregnant dams and for evaluation of reproductive toxicity and risk assessment of new drug candidates.
the natural mating method, which cohabited male and female monkeys in a cage and produced mated animals. As a result of three days’ natural mating, 15 out of 18 female animals (83%) succeeded in mating, and 8 out of the mated ones (53%) succeeded in pregnancy. Mating was confirmed through observing the mating behavior and checking sperm in vagina smear. Pregnancy diagnosis and observation of embryonic development were performed using ultrasonic diagnostic equipment. According to the results of the tests, the endometrial cavity was opened by around a third on Day 18 of pregnancy, and the egg yolk and heart rate of the endometrial cavity were observed from Day 25. The fetal form was observed to some degree on Day 35, and the completed form of fetus was observed on Day 45. Caesarean section was conducted on Day 50 of pregnancy. To anesthetize pregnant animals, we performed intramuscular injection of tranquilizer, which was 0.1 mg/kg of medetomidine. We also applied intramuscular injection of 10 mg/kg of ketamine as an anesthetic and maintained oxygen and 1-2% of isoflurane using a mask to maintain anesthesia during operation. The condition of the gravid uterus, the survival and the external examination of the fetus were observed. In conclusion, the natural mating method established in this study was found to be a useful tool for obtaining pregnant dams and for evaluation of reproductive toxicity and risk assessment of new drug candidates.
목차
재료 및 방법
결과
고찰
감사의 글
참고문헌
참고문헌 (18)
참고문헌 신청
1. [학술지(정기간행물)] - Brown, N.A. / 1983 / value of animal teratogenicity testing for predicting human risk / Clin. Obstet. Gynecol 26 : 467 ~ 477
2. [학술지(정기간행물)] - Chung, M.K. / 2000 / Principles and methods for the reproductive-toxicological of new drug candidates / J. Toxicolpub. Health 16 : 229 ~ 238
3. [학술지(정기간행물)] - Einspanier, A. / 2006 / The common marmoset monkey as a model for implantation and early pregnancy research / Methods Mol. Med 121 : 111 ~ 121
4. [단행본] - Fortman, J.D. / 1999 / The Laboratory Nonhuman Primate / CRC Press : 35 ~ 40
5. [학술지(정기간행물)] - Herodin, F. / 2005 / Nonhuman primates are relevant models for research in hematology, immunology and virology / Eur. Cytokine Netw 16 : 104 ~ 116
2. [학술지(정기간행물)] - Chung, M.K. / 2000 / Principles and methods for the reproductive-toxicological of new drug candidates / J. Toxicolpub. Health 16 : 229 ~ 238
3. [학술지(정기간행물)] - Einspanier, A. / 2006 / The common marmoset monkey as a model for implantation and early pregnancy research / Methods Mol. Med 121 : 111 ~ 121
4. [단행본] - Fortman, J.D. / 1999 / The Laboratory Nonhuman Primate / CRC Press : 35 ~ 40
5. [학술지(정기간행물)] - Herodin, F. / 2005 / Nonhuman primates are relevant models for research in hematology, immunology and virology / Eur. Cytokine Netw 16 : 104 ~ 116
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