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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.12
- 수록면
- 609 - 616 (8page)
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The occurrence of the avian bird flu can be very harmful not only poultry industry but also human life and health. To solve these problems, Jeju native chicken, which has not been taken avian influenza disease until right now in Korea, was used. This study verified the possibilities of antiviral activity against avian influenza using method of DNA polymorphism of the Mx gene known as the anti virus gene and the part of specific gene. Also, some specific sites were used for expression vector construction and those vectors were transfected into chicken embryonic fibroblast cells for the confirmation of antiviral activity. As a result, common specific variations were found in 21 spots in Jeju native chicken. The part of UTR region was expressed intensively into embryonic fibroblast cells of each experimental strain. Antiviral activity was expressed highly as 29% in Jeju native chicken strain than any other commercial strain. Mx gene which was selected by Jeju native chicken was also expressed in spermatogonia stem cells that would be used for the next generation. We expect that this study will be used as an important material when we select anti virus chicken and study gene manipulation.
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재료 및 방법
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고찰
감사의 글
참고문헌
참고문헌 (29)
참고문헌 신청
1. [학술지(정기간행물)] - Bernasconi, D. / 1995 / The interferon-induced Mx protein of chickens lacks antiviral activity / J. Interferon Res 15 : 47 ~ 53
2. [학술지(정기간행물)] - Claas, E.C. / 1998 / Human influenza a H5N1 virus related to a highly pathogenic avian influenza virus / Lancet 351 : 472 ~ 477
3. [학술지(정기간행물)] - Clemens, M.J. / 1997 / The double-stranded RNAdependent protein kinase PKR: structure and function / J. Interferon Cytokine Res 17 : 503 ~ 524
4. [학술지(정기간행물)] - Das, R.M. / 2006 / A robust system for RNA interference in the chicken using a modified microRNA operon Develop / Biol 294 : 554 ~ 563
5. [학술지(정기간행물)] - Dnyaneshwar, W. / 2006 / Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN / Biol. Pharm. Bull 29 : 2313 ~ 2316
2. [학술지(정기간행물)] - Claas, E.C. / 1998 / Human influenza a H5N1 virus related to a highly pathogenic avian influenza virus / Lancet 351 : 472 ~ 477
3. [학술지(정기간행물)] - Clemens, M.J. / 1997 / The double-stranded RNAdependent protein kinase PKR: structure and function / J. Interferon Cytokine Res 17 : 503 ~ 524
4. [학술지(정기간행물)] - Das, R.M. / 2006 / A robust system for RNA interference in the chicken using a modified microRNA operon Develop / Biol 294 : 554 ~ 563
5. [학술지(정기간행물)] - Dnyaneshwar, W. / 2006 / Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN / Biol. Pharm. Bull 29 : 2313 ~ 2316
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UCI(KEPA) : I410-ECN-0101-2009-510-015707111