지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.49 No.4
- 발행연도
- 2008.8
- 수록면
- 258 - 263 (6page)
이용수
초록· 키워드
오류제보하기
Beta amyloid (Aβ) is a potent oral immunogen with potential utility as a vaccine against Alzheimer's disease (AD). Our previous work investigated the ability of a protein comprised of five tandem repeats of Aβ42(5Aβ42) to induce an immune response in a transgenic AD mouse model, and demonstrated that immunization with potato-derived 5Aβ42 reduced the Aβburden as well as many of the AD-associated pathological features. To provide a potato-derived 5Aβas vaccine material, the stable yield of 5Aβprotein through constant expression of introduced transgene is the most important factor. In the present study, we confirmed that the 5Aβ42 transgene were inherited and stably expressed in in vitro vegetative propagation over a period of 3 years without silencing. The 5Aβ42 protein, too, was expressed well without change of its antigenicity in transgenic potato. Stable storage conditions were vital in maintaining the stability of the potato-derived 5Aβ42 protein, and activity of the protein was not affected following up to three months’ storage at low temperature.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Literature Cited
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Borkowska, M. / 1995 / Transformation of diploid potato with an Agrobacterium tumefaciens binary vector system. Ⅱ. Stability of transformation in tubers, micropropagated and greenhouse grown plants / Acta Physiol. Plant 17 : 275 ~ 280
2. [학술지(정기간행물)] - Chikwamba, R. / 2002 / A functional antigen in a practical crop: LT-B producing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and cholera toxin (CT) / Transgenic Res 11 : 479 ~ 493
3. [학술지(정기간행물)] - Chikwamba, R.K. / 2003 / Localization of bacterial protein in starch granules of transgenic maize kernels / Proc. Natl. Acad. Sci 100 : 11127 ~ 11132
4. [학술지(정기간행물)] - Gursky, O. / 2000 / Temperature-dependent β-sheet formation inβ-amyloid Aβ1-40 peptide in water: uncoupling -structure folding from aggregation / Biochim. Biophys. Acta β1 476 : 93 ~ 102
5. [학술지(정기간행물)] - Huang, Z. / 2005 / Virus-like particle expression and assembly in plants: hepatitis B and Norwalk viruses / Vaccine 23 : 1851 ~ 1858
2. [학술지(정기간행물)] - Chikwamba, R. / 2002 / A functional antigen in a practical crop: LT-B producing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and cholera toxin (CT) / Transgenic Res 11 : 479 ~ 493
3. [학술지(정기간행물)] - Chikwamba, R.K. / 2003 / Localization of bacterial protein in starch granules of transgenic maize kernels / Proc. Natl. Acad. Sci 100 : 11127 ~ 11132
4. [학술지(정기간행물)] - Gursky, O. / 2000 / Temperature-dependent β-sheet formation inβ-amyloid Aβ1-40 peptide in water: uncoupling -structure folding from aggregation / Biochim. Biophys. Acta β1 476 : 93 ~ 102
5. [학술지(정기간행물)] - Huang, Z. / 2005 / Virus-like particle expression and assembly in plants: hepatitis B and Norwalk viruses / Vaccine 23 : 1851 ~ 1858
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UCI(KEPA) : I410-ECN-0101-2009-525-011286690