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자료유형
학술저널
저자정보
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.24 No.3
발행연도
2008.9
수록면
328 - 336 (9page)

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초록· 키워드

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Out of various fungi and bacteria tested for inhibition of Tobacco mosaic virus (TMV) infection using Nicotiana tabacum cv. Xanthi-nc, a bacterial isolate JB-1, identified as Pseudomonas putida had a strong direct inhibitory activity against the TMV infection. Its systemic or indirect activity was also noted at more than a half level of the direct control efficacy. Disease severity was reduced significantly in the susceptible tobacco N. tabacum cv. NC 82 by the treatment of the bacterial culture filtrate, somewhat more by the pretreatment than by simultaneous treatment, probably by inhibiting the TMV transmission and translocation in the plants, showing negative serological, which responses in the viral detection by DAS-ELISA. TMV-inhibitory substances from P. putida JB-1 were water-soluble, stable to high temperature (even boiling), and to a wide range of pH. As proteinase K nullified their antiviral activity, the TMV inhibition activity of P. putida may be derived from proteinaceous materials. In electron microscopy, TMV particles treated with the JB-1 culture were shown to be shrunken with granule-like particles attached on them. All of these aspects suggest that P. putida JB-1 may be developed as a potential agent for the control of TMV.

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