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Most biodosimetric studies of unanticipated radiation exposure fail to specify cause and effect because researchers have not been able to quantity a valid dosage for many victims in multi-casualties. To date, there has not been an effective technique responsible for all the requirements of ideal biodosimetry. In order to determine whether the apoptosis induction assay could be used to estimate an early dose prediction of the whole body or a part of it within 12 h after accidental radiation exposure, we examined apoptotic lymphocytes after ??Co γ-rays within the dose range of 0.25 to 1 Gy. Apoptotic cell death rose steeply at low doses up to 1 Gy and radiation induced rapid changes in peripheral lymphocytes at all dose levels. These data suggest that apoptosis may play an important role in the homeostasis of radiosensitive target organs by the removal of damaged cells. The dose-response curves were linear-quadratic between the frequency of apoptotic lymphocytes and the dose. In addition, there were significant peaks for apoptosis at 4 and 6 h after irradiation, and the morphological findings of the irradiated lymphocytes were typical apoptotic cells that were rarely observed in the non-irradiated controls.

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UCI(KEPA) : I410-ECN-0101-2009-510-016356136