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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.48 No.6
발행연도
2007.12
수록면
387 - 392 (6page)

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Adventitious shoot regenerations of chrysanthemum cultivars ‘Klondike’, ‘Pink Pixie Time’, and ‘Orlando’ were achieved through culture of petal explants on the Murashige and Skoog medium supplemented with B5 vitamins and plant growth regulators. Effect of different concentrations and combinations of IAA, BAP, and kinetin and explant ages was investigated. Petals were collected at two stages of flower development. Stage I petals were collected at 2~3 days before ray flowers were fully open, while stage II petals were collected at 7~8 days after tubular flowers opened. Explants from stage I were more organogenic than those from stage II. The highest regeneration frequency was obtained by culturing stage I petal explants in the dark for 14 days, followed by culturing for 16 days at 25±1 under ℃ a 16 h photoperiod (40 μmolㆍm?²ㆍs?¹) on the Murashige and Skoog medium supplemented with 57.0 μM IAA, 44.0 μM BAP and 0.4 μM kinetin. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the stock plant.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
Literature Cited

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