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To develop an efficient embryogenic culture in satsuma mandarin, some factors related to physiological and environmental conditions were investigated during embryogenic culture initiation from undeveloped seeds of ‘Miyagawa Wase’ and ‘Okitsu Wase’ satsuma mandarin. Much more undeveloped seeds were obtained from larger fruits and the number of large seeds was positively correlated with fruit size. However, large seeds showed lower frequency in initial callus induction and somatic embryogenesis. Effects of culture condition, medium type, carbohydrate source, and agar concentration on initial callus induction and embryogenesis were further evaluated. There was no effect of culture condition of darkness or light. MT and l/2EME media were effective in ‘Miyagawa Wase’ and ‘Okitsu Wase’, respectively. In the experiment of carbohydrate source, 3% sucrose was more effective than 5% sucrose or 2% glycerol. In addition, agar concentration higher than 1.2% was favorable to callus induction in both cultivars.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2009-525-016949389