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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.2
발행연도
2005.4
수록면
119 - 125 (7page)

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In vitro plantlets of potato genotype ‘F9-99’ infected with PLRV and PVY were used for the virus eradication using heat and chemical treatments. Subculture medium was added with acetylsalicylic acid (ASA) (10??, 10??, and 10?⁴ M) and ribavirin (10, 15, and 20 ㎎ㆍL?¹) alone or in combinations. The plantlets were incubated at 25℃ (control), and alternating heat treatments 35/30℃ for 1, 4, and 8 h or 42/25℃ for 4 h. Virus status of the plantlets were examined by means of double antibody sandwich-enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction. After 4?8 weeks of treatment, 40, 51, 41, 36, and 0% survival were observed in the control (MS and 25℃), ASA plus ribavirin (25℃), MS plus heat treatment (35/30℃), ASA plus ribavirin (35/30℃), and MS medium with heat treatment (42/25℃), respectively. As for virus eradication, heat treatment (35/30℃) with 1 h alternating thermo-cycle resulted in 100% PVY elimination, but it was much more difficult to remove the PLRV from the double-infected potato plantlets with PVY and PLRV. The highest rate of PLRV eradication was 60% from the combined treatment of 10?? M ASA, 20 ㎎ㆍL?¹ ribavirin, and heat treatment at 35/30℃ for 8 h alternating temperature.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited

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