지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The chicken liver has been involved in various biological functions including detoxification, glycogen storage and plasma protein synthesis. The aim of this study was to investigate differentially expressed genes in chicken liver in four different growing stages. Using 10 arbitrary Annealing Control Primers (ACPs), five differentially expressed genes have been identified. Based on the Basic Local Alignment Search Tool (BLAST) search results, three of them were matched with previously known genes, and the other two were matched with unknown EST sequence and a hypothetical protein, respectively. In order to confirm the expression results, quantitative real-time PCR was also performed. The high similarities between the expression data using arbitrary ACPs and quantitative real-time PCR indicate that the identified genes are the real differentially expressed genes in different growing stages. The genes identified in this study can be used as valuable biomarkers in chicken with further investigation of the functions.
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ABSTRACT
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재료 및 방법
결과 및 고찰
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참고문헌 (20)
참고문헌 신청
Ahmed FE , 2002 , Molecular techniques for studying gene expression in carcinogenesis 20 (2) : 77 ~ 116
Arnon S , 2007 , Serum amyloid A: An early and accurate marker of neonatal early-onset sepsis
Choi DH , 2002 , Expression of a novel 90 kDa heat shock protein in colorectal tumor 18 (1) : 1 ~ 6
Cui XS , 2005 , Identification of differentially expressed genes in murine embryos at the blastocyst stage using annealing control primer system 70 (3) : 278 ~ 287
Godfroid E , 1998 , Detection and identification of human papilloma viral DNA, types 16, 18, and 33, by a combination of polymerase chain reaction and a colorimetric solid phase capture hybridisation assay 75 (1) : 69 ~ 81
Arnon S , 2007 , Serum amyloid A: An early and accurate marker of neonatal early-onset sepsis
Choi DH , 2002 , Expression of a novel 90 kDa heat shock protein in colorectal tumor 18 (1) : 1 ~ 6
Cui XS , 2005 , Identification of differentially expressed genes in murine embryos at the blastocyst stage using annealing control primer system 70 (3) : 278 ~ 287
Godfroid E , 1998 , Detection and identification of human papilloma viral DNA, types 16, 18, and 33, by a combination of polymerase chain reaction and a colorimetric solid phase capture hybridisation assay 75 (1) : 69 ~ 81
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UCI(KEPA) : I410-ECN-0101-2009-527-016772346