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자료유형
학술저널
저자정보
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.20 No.2
발행연도
2004.6
수록면
131 - 134 (4page)

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초록· 키워드

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A strain of Cucumber mosaic virus (CMV) was isolated from a weed, water chickweed (Stellaria aquatica), growing in the pepper field in Chunchon, Korea. This isolate, CMV-Sa, was differentiated from other CMVs based on biological properties and nucleotide sequence analysis of the coat protein (CP) gene. CMV-Sa showed different reactions to all the tested plants, except Capsicum annuum and Cucumis sativus, when compared with those of CMV-Mf (subgroup Ⅰ) and CMV-PaFM (subgroup Ⅱ). Remarkably, in Nicotiana tabacum cvs. Samsun, Xanthi-nc and Ky-57, CMV-Sa induced local necrotic ring spots on the inoculated leaves and venal wave pattern and mosaic on the upper leaves. RNA analysis, serology, and RT-PCR of CP gene showed that CMV-Sa belonged to subgroup Ⅰ of CMV. However, restriction enzyme analysis of the cDNA using AluⅠ, HhaⅠ, HincⅡ, HindⅢ, HinfⅠ and MspⅠ showed that CMV-Sa was distinct from that of CMV-Mf. Based on comparison of the nucleotide of CP gene and deduced amino acid sequences between other CMV strains, CMV-Sa was closely related to CMV-Mf with 93.7% and 97.2% identity, respectively.

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Materials and Methods
Results and Discussion
Acknowledgements
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UCI(KEPA) : I410-ECN-0101-2009-481-017567304