지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.6
- 수록면
- 127 - 130 (4page)
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초록· 키워드
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Grapevine leaf roll-associated virus 3 (GLRa V-3) is one of the most important viral diseases of grapevine in the world. In this study, GLRa V-3 Ampelovirus was identified from grapevines in Korea by analyzing viral coat protein size, nucleotide, and amino acid sequences. The molecular weight of viral coat protein from virus￢infected in vitro plantlets was determined by western blot using a commercial GLRa V-3 polyclonal antibody. Western blot analysis showed a coat protein of about 43 kDa. RT-PCR product of about 942 bp which encoded the coat protein (CP) gene was amplified with specific primers. When the viruses existed at low titers in the host plant, the dsRNA had very specific template in RT-PCR amplification of fruit tree viruses. Especially, small-scale dsRNA extraction method was very reliable and rapid. Sequence analysis revealed that the CP of the GLRa V-3 Ko consisted of 942 bp nucleotide, which encoded 314 amino acid residues. The CP gene of GLRa V-3 Ko had 98.9% nucleotide sequence and 98.7% amino acid sequence identities with earlier reported GLRa V-3. This is the first report on molecular assay of GLRa V-3 Ampelovirus identified from Korea. The GLRa V-3 Ko CP clone would be very useful for breeding of virus resistant grapevines.
목차
Materials and Methods
Results and Discussion
References
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UCI(KEPA) : I410-ECN-0101-2009-481-017567293