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자료유형
학술저널
저자정보
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.18 No.6
발행연도
2002.12
수록면
317 - 322 (6page)

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초록· 키워드

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An isolate of Odontoglossum rings pot virus (ORSV) was identified from Cymbidium var. ‘Grace Kelly’ showing ringspot symptom on the floral and leaf parts, and was denoted as cymbidium ringspot isolate (ORSV-CR). In ultrathin sections of leaf tissue from diseased Cymbidium plants, clusters of virus particles were observed in the vacuole and cytoplasm. In the Western blot hybridization, the virus strongly reacted with ORSV-specific antiserum indistinguishable from ORSV, suggesting that the virus is serologically identical with ORSV. ORSV-CR sap was inoculated onto 20 species belonging to 12 genera. Systemic infection occurred in Cymbidium sp., Nicotiana benthamiana and N. cievelandii, the host of which was found to be different from that ofORSV-Cy, the Korean strain of ORSV. The analysis of coat protein (CP) gene showed that ORSV-CR was highly homologous to the known isolates of ORSV, with over 95.6% identity in amino acid level. Phylogenetic tree analysis of CP showed that ORSV-CR was clustered with the known ORSV isolates, suggesting that ORSV is a very stable tobamovirus.

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Materials and Methods
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UCI(KEPA) : I410-ECN-0101-2009-481-017566589