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학술저널
저자정보
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.16 No.2
발행연도
2000.4
수록면
98 - 104 (7page)

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초록· 키워드

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For the taxonomic evaluation, 15 strains of the genus Pectobacterium and Erwinia were analyzed for 16S-23S rDNA intergenic spacer regions (ISRs). These species contained two types of ISRs, large and small ISRs. Large ISRs were on the range of 474-569 bp size, and coding transfer RNA<SUP>Ile</SUP> (tRNA<SUP>Ile</SUP>) and tRNA<SUP>AlA</SUP>. Small ISRs were 354-459 bp in length and coding tRNA<SUP>Glu</SUP>. The sequence variations of two ISRs among species and strains were very high as compared with 16S rRNA gene sequences. By phylogenetic trees on the basis of two ISRs, Pectobacterium were differentiated into P. carotovorum-P. cacticidum group and P. chrysanthemi group. However, the taxonomic position of E. cypripedii and E. rhapontici, which were not clear on taxonomic delineation between Pectobacterium and Erwinia, were not clearly resolved on the basis of ISRs.

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Materials and Methods
Results and Discussion
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UCI(KEPA) : I410-ECN-0101-2009-481-017559799