지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2000.8
- 수록면
- 189 - 199 (11page)
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초록· 키워드
오류제보하기
To search for the antifungal substances, various actinomycete isolates were obtained from various soils of Korea using plate dilution method on the humic acid vitamin agar plates. In the screening procedures using a dual culture method, 32 actinomycete isolates were selected, which showed the inhibitory activity against mycelial growth of plant pathogenic fungi Alternaria mali, Colletotrichum gloeosporides, Fusarium oxysporum f.sp, cucumerinum, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. Bioassay of the crude extracts from culture filtrates and mycelial mets revealed that 12 antagonistic actinomycetes produced highly active antifungal substances. Actinomycete strain S5-55 which showed the substantial antifungal activity against the tested fungi was selected for production of the antifungal substances. Based on the cytochemical and morphological characteristics, strain S5-55 was identified as a Streptomyces species. The results of the numerical identification using the TAXON program confirmed that Streptomyces strain S5-55 was identical with Streptomyces humidus including in TAXON major cluster 19. The production of antifungal substance was most favorable when S. humidus strain S5-55 was cultivated for 10 days on soluble starch broth supplemented with K₂HPO₄. The antifungal substances active against the plant pathogenic fungi P. capsici and M. grisea were partially purified using C₁? reversed-phase column chromatography.
목차
Materials and Methods
Results
Discussion
Acknowledgement
References
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UCI(KEPA) : I410-ECN-0101-2009-481-017559522