고추 疫病을 效果的으로 防除하기 위해 拮抗菌을 土壤 鹽類濃度와 處理時期를 달리하여 生物學的 防除效果를 檢定하였다. 發病 抑制型 土壤에서 分離된 拮抗菌중 室內 檢定한 結果, 優秀한 菌은 Trichoderma spp., T. harzianum, T. hamatum, Bacillus lentus, Enterobacter agglomerans, Pseudomonas mendocina 로 同定되었고, 그 중 病原菌에 대한 拮抗作用과 不良環境 (EC, pH)에 대한 耐性이 가장 優秀한 T. harzianum T873, T. hamatum T77, E. agglomerans B2를 고추疫病의 防除에 使用하였다. 土壤鹽類濃度 EC 1, 3및 5 (mS/㎝)로 각각 調節된 土壤에서 고추 疫病의 罹病率은 52%, 43%, 21%로 나타났고, 土壤鹽類濃度에 관계없이 拮抗菌의 發病抑制效果는 비교적 크게 나타났다. 특히 T. harzianum T873의 無處理에 비해 크게 나타났고, EC 5에서 고추의 生育, 특히 뿌리의 發育이 다소 不良했으나 4%의 낮은 罹病率을 나타내었다. 室內 實驗에서 拮抗力이 가장 優秀했던 拮抗곰팡이(T. harzianum T873)를 고추 移植期別로 處理하여 病發生 抑制效果를 調査한 結果 고추 60일苗를 본포에 定植할 때 土壤處理 하는 것 보다는, 고추育苗를 위해 20일 苗를 pot에 移植할때 pot土壤에 섞어 處理하는 것이 發病抑制效果가 뚜렷하였다. 그러나 拮抗細菌인 E. agglomerans의 경우, 室內에서 拮抗菌은 優秀했으나, 포장 처리에서는 두 方法 모두 發病抑制效果를 기대할 수 없었다.
Six antagonists isolated from suppressive soils to pepper crown and root rot caused by Phytophthora capsid at southern greenhouse area were identified as Trichoderma spp., T. harzianum, T. hamatum, Bacillus lentus, Enterobacter agglomerans, and Pseudomonas mendocina. The most promising three antagonists were T. harzianum T873, T. hamatum T77, and Enterobacter agglomerans in vitro tests. Disease incidence was reduced when the soil electrical conductivity (EC) was increased to EC 3 and 5. However, root growth was retarded at EC 5 soil. Application of T. harzianum T873 significantly reduced disease incidence at all levels of soil EC tested. This isolate was selected for its tolerance over wide range of adverse soil environments as well as antagonistic activity such as overgrowing and lysis of P. capsici. Disease incidences in antagonist-treated soil vs. non-treated soil were 9% vs. 52%, 18% vs. 40%, and 4% vs. 23% at EC 1, 3, and 5 (mS/㎝) respectively. The selected antagonistic bacterium, E. agglomerans B2, and the fungus, T. harzianum T873 were applied by two different methods for disease control. First, biological control agents were introduced to nursery pot soil, where two-week old seedlings were planted (pot-treatment) and after two months, these seedlings were transplanted to the field soil. Secondly, antagonists were introduced in field soil just before transplanting of the two-month old pepper seedlings (field-treatment). Comparing pot vs. field treatment of antagonists, pot treatment of T. harzianum T873 was more promising for remarkably effective antagonistic activity than field treatment. Although E. agglomerans B2 was strongly antagonistic in vitro, it was not effective to control the disease in field with both treatments. The possible mechanisms involved are discussed.