韓國의 蘭栽培地域 바이러스 發生을 調査하기 위하여 1985년부터 1989년까지 5年동안 13屬 52種에 속하는 640株의 蘭科植物로부터 바이러스를 分離하여 몇 가지 바이러스의 특성을 調査한 結果 Dendrobium mosaic virus(DMV), Odontoglossum ringspot virus(ORSV). Orchid fleck virus(OFV), 未同定 potyvirus 등 4種의 바이러스를 추가적으로 發見하였다. 大邱 蘭栽培地의 덴드로비움에서 分離한 DMV는 DN(direct negative staining)法에 의하여 電子顯微鏡으로 觀察한 結果 길이 750㎚의 絲狀粒子로 超薄片切斷에 의해 感染 葉肉細胞의 細胞質에서 絲狀形의 바이러스粒子와 細胞質封入體가 관찰되었다. ORSV는 48個 地域의 나도풍란, 새우란, 심비디움, 덴드로비움 등 10屬에서, OFV는 대구와 왜관에서 採集된 심비디움, 덴드로비움, 온시디움 등 6屬에서 分離되었고, 各各의 바이러스는 약 300㎚ 以內의 稈狀粒子와 40×150㎚의 短稈形粒子로 觀察되었다. ORSV의 경우 罹病葉肉細胞에서 바이러스 粒子와 結晶封入體와 X-material이 觀察되었다. OFV에 感染된 담배의 罹病細胞에서는 核內 바이로플라즘이 罹病初期에 形成되어 이것이 점차적으로 發達하여 核周圍와 內部에 短稈形의 粒子와 粒子集團이 集積되고 核膜을 통하여 細胞質로 轉移되는 모양과 細胞質내에서는 膜에 둘러싸인 粒子集團이 觀察되었다. 길이 780㎚의 未同定 potyvirus는 光州地域 野山에서 採集된 모자이크 病狀의 春蘭에서 分離되었으며, 細胞質과 液胞에 약 780㎚의 絲狀粒子 集團과 細胞質封入體가 확인되었으며, 진딧물에 의해 쉽게 傳染되는 특징을 나타내었다. 汁液接種에 의해 各各의 바이러스는 수종의 檢定植物에 感染되어 全身 또는 局部病徵을 나타내었다. CyMV와 ORSV는 韓國 蘭栽培의 全地域의 我栽蘭에서 檢出되었고, 被害도 가장 심하였다. ORSV는 組織培養에서 얻어진 幼苗에서도 分離되었다. CyMV와 ORSV, CyMV와 CMV, CyMMV와 ORSV. DMV와 CMV의 混合感染이 새우란, 심비디움, 덴드로비움 등 7屬에서 확인되었다.
In the survey of virus diseases of orchids in Korea from 1985 to 1987, investigating 640 orchid plants in 52 species of 13 genera, 4 additional viruses such as dendrobium mosaic virus (DMV), odontoglossum ringspot virus (ORSV), orchid fleck virus (OFV) and an unidentified potyvirus were found, which were identified depending on several characteristics. Electron microscope by using direct negative staining method revealed DMV particles with filamentous shape of about 750 ㎚ in length, which were isolated from Dendrobium collected from orchid production area in Taegu. In the ultra-thin sections of the infected tissues, cylindrical unclusions and virus particles were found in the mesophyll cells. ORSV and OFV, isolated from 10 genera of orchid plants including Aerides; Cymbidium, and Dendrobium in 48 areas of the orchid production region, and 6 genera of orchid plants including Cymbidium and Dendrobium in Taegu and Waegan, were rod shape of about 300 ㎚ in length and bacilliform of 45×150 ㎚ in size, respectively. In ORSV, aggregates and crystalline arrays of virus particles and X-material were found in the cytoplasm, nuclei, and vaculoes of infected mesophyll cells. In OFV infected tissues, a number of virus particles were found in the nuclei and in the cytoplasm, and the viroplasm development was also observed, indicated by formation and accumulation of viroplasm and virus particles in the nucleus and around nuclear envelope, disperse of virus particles through the nuclear envelop and accumulation of virus particles in the cytoplasm. The identified potyvirus with 780 ㎚ in length was isolated from cymbidium goeringii with mosaic symptom in Kwangju. In the infected plants, virus particles of about 730 ㎚ in length and typical cylindrical inclusions were found. The virus was easily transmitted by Myzus persica as a non-persistent manner. Host range of each isolated virus was examined using several test plants. Double infection of CyMV and ORSV, CyMV and CMV, CyMMV and ORSV, DMV and CMV was found in Calanthe, Cattleya, Cymbidium, Dendrobium; Oncidium; Phaius, and Phalaenopsis.