지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.9
- 수록면
- 211 - 217 (7page)
이용수
초록· 키워드
오류제보하기
For discrimination of ductal and ascinar cells, we isolated a single-chain variable domain fragment (scFv) antibody against ductal cells of salivary gland using phage display technique. From the spleen of a mouse immunized with ductal cell lysate, total RNA was prepared and used as a template for cDNA synthesis of antibody genes. The scFv genes were constructed with variable domain genes of heavy and light chain and Were introduced into pCANTAB5E to construct phage scFv library. The phage particles specific for acinar cells were screened by subtraction using immunotubes coated with acinar and ductal cell lysate and enzyme-linked immunoabsorbance assay (ELISA). The characteristics of the scFv were determined by immunohistochemistry (IHC) and the result indicated that the isolated scFv has the specificity against ductal cells of salivary glands and tubules of kidney. And the scFv has an unique binding activity specific for Hashimoto's thyroiditis. The nucleotide sequence of isolated scFv gene was determined and revealed that V<SUB>H</SUB> belongs to the mouse H-chain family subgroup IB and V<SUB>L</SUB> to the mouse L-chain family subgroup Ⅲ.
목차
영어 초록
서론
재료 및 방법
결과
고찰
감사의 글
REFERENCES
참고문헌 (11)
참고문헌 신청
[학술지(정기간행물)] - Einfeld DA, / 1998 / Molcular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains. 7 : 711 ~ 717
[학술지(정기간행물)] - Gao C, / 2003185-197 / De novo identification of tumor-specific internalizing human antibody-receptor pairs by phage-display methods. 274 : 185 ~ 197
[학술지(정기간행물)] - Hoogenboom HR, / 1999 / Selection-dominant and nonaccessible epitopes on cell-surface receptors revealed by cell-panning with a large phage antibody library. 260: : 774 ~ 784
[학술지(정기간행물)] - Huston JS / 2001 / Engineered antibodies take cancer stage. 10 : 127 ~ 142
[학술지(정기간행물)] - Jesper T, / 1997 / Efficient selection of scFv antibody phage by adsorption to in situ expressed antigens in tissue sections. 210 : 11 ~ 23
[학술지(정기간행물)] - Gao C, / 2003185-197 / De novo identification of tumor-specific internalizing human antibody-receptor pairs by phage-display methods. 274 : 185 ~ 197
[학술지(정기간행물)] - Hoogenboom HR, / 1999 / Selection-dominant and nonaccessible epitopes on cell-surface receptors revealed by cell-panning with a large phage antibody library. 260: : 774 ~ 784
[학술지(정기간행물)] - Huston JS / 2001 / Engineered antibodies take cancer stage. 10 : 127 ~ 142
[학술지(정기간행물)] - Jesper T, / 1997 / Efficient selection of scFv antibody phage by adsorption to in situ expressed antigens in tissue sections. 210 : 11 ~ 23
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Generation of 1E8 Single Chain Fv-Fc Construct Against Human CD59
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타액선 도관세포의 관류 배양 기술 개발
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유방 관상피내암(Ductal carcinoma in situ: DCIS)의 진단과 치료의 특성
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하시모토 갑상선염을 동반한 갑상선 결절 환자의 임상적 특징
Annals of Surgical Treatment and Research
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관상피내암의 임상고찰 및 병리조직학적 분석
Annals of Surgical Treatment and Research
1997 .03
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Association Kikuchi disease with Hashimoto thyroiditis: a case report and literature review
Annals of Pediatirc Endocrinology & Metabolism
2018 .01
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폐쇄성 타액선염을 일으키는 드문 타액선관 질환들에서 타액선 내시경술의 유용성
대한이비인후-두경부외과학회지
2008 .01
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하시모토 갑상선염에 동반된 갑상선암에 대한 임상적 고찰
Annals of Surgical Treatment and Research
1997 .05
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Microsatellite Instability in Invasive Ductal Carcinomas
대한외과학회 학술대회 초록집
2006 .11
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UCI(KEPA) : I410-ECN-0101-2009-510-017341722