지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2001.12
- 수록면
- 161 - 166 (6page)
이용수
초록· 키워드
오류제보하기
Most mammalian cells take up glucose by passive transport proteins in the plasma membranes. The best known of these proteins is the human erythrocyte glucose transporter, GLUT1. High levels of heterologous expression for the transporter are necessary for the investigation of its three-dimensional structure by crystallization. To achieve this, the baculovirus expression system has become popular choice. However, Spodoptera frugiperda Clone 9 (Sf9) cells, which are commonly employed as the host permissive cell line to support baculovirus replication and protein synthesis, grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, suggesting the presence of endogenous glucose transporters. Furthermore, very little is known of the endogenous transporters properties of Sf9 cells. Therefore, human GLUT1 antibodies would play an important role for characterization of the GLUT1 expressed in insect cell. However, the successful use of such antibodies for characterization of GLUT1 expression in insect cells relies upon their specificity for the human protein and lack of cross-reaction with endogenous transporters. It is therefore important to determine the potential cross-reactivity of the antibodies with the endogenous insect cell glucose transporters. In the present study, the potential cross-reactivity of the human GLUT1 antibodies with the endogenous insect cell glucose transporters was examined by Western blotting. Neither the antibodies against intact GLUT1 nor those against the C-terminus labelled any band migrating in the region expected for a protein of M, comparable to GLUT1, whereas these antibodies specifically recognized the human GLUT1. Specificity of the human GLUT1 antibodies tested was also shown by cross-reaction with the GLUT1 expressed in insect cells. In addition, the insect cell glucose transporter was found to have very low affinity for cytochalasin B, a potent inhibitor of human erythrocyte glucose transporter.
목차
영어 초록
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
생쥐 초기배아의 Glucose Transporter유전자 발현 양상에 관한 연구
대한불임학회잡지
1998 .01
-
담낭암에서 당 수송단백 GLUT1의 발현양상
Annals of Hepato-Biliary-Pancreatic Surgery
2000 .12
-
Hexose Uptake and Kinetic Properties of the Endogenous Sugar Transporter(s) in Spodoptera frugiperda Clone 21-AE Cells
대한의생명과학회지
2005 .09
-
Glucose Transporter-1 Expression in Squamous Cell Carcinoma of the Tongue
Cancer Research and Treatment
2007 .01
-
The Uptake of 2-deoxy-D-glucose (2dGlc) by the Endogenous Sugar Transporter(s) of Spodoptera frugiperda CLone 21-AE Cells and the Inhibition of 2dGlc Transport in the Insect Cells by Fructose and Cytoc halasin B
대한의생명과학회지
2003 .12
-
Photoaffinity Labelling of the Human Erythrocyte Glucose Transporters Expressed in Spodoptera frugiperda Clone 9 (Sf9) Cells
대한의생명과학회지
2002 .12
-
Glucose Transporters and Insulin Action : Some Insights into Diabetes Management
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1999 .01
-
유방암조직에서 당 수송단백 GLUT1의 발현 양상과 유방암 예후 인자와의 상관관계
Annals of Surgical Treatment and Research
2001 .05
-
Immunohistochemical Evaluation of Glucose Transporter Type 1 in Epithelial Dysplasia and Oral Squamous Cell Carcinoma
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Differential Expression of Glut1 in Pulmonary Neuroendocrine Tumors: Correlation with Histological Grade
Journal of Pathology and Translational Medicine
2009 .01
-
Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin
대한생리학회지
1993 .01
-
악성종양의 포도당 섭취 기전
대한핵의학회지
1999 .01
-
Tryptic Digestion and Cytochalasin B Binding Assay of the Human HepG2-Type Glucose Transporter Expressed in spodoptera frugiperda Clone 21-AE Cells
대한의생명과학회지
2005 .03
-
침윤성 유방암종에서 예후인자로서의 GLUT1 단백질의 발현
Annals of Surgical Treatment and Research
2001 .09
-
A Study on the Inhibition of 2-deoxy-D-Glucose Transport of the Endogenous Glucose Transporters in Spodoptera frugiperda Clone 21-AE Cells by Using Hexoses
대한의생명과학회지
2005 .12
-
Molecular Association of Glucose Transporter in the Plasma Membrane of Rat Adipocyte
대한생리학회지
1991 .01
-
Toxicological Relevance of Transporters
Toxicological Research
2007 .03
-
GLUT1 as a Prognostic Factor for Classical Hodgkin’s Lymphoma: Correlation with PD-L1 and PD-L2 Expression
Journal of Pathology and Translational Medicine
2017 .01
-
두경부 편평 상피 세포암에서 Glucose Uptake Transporter-1과 Epidermal Growth Factor Receptor의 발현 양상
대한이비인후-두경부외과학회지
2009 .01
-
사람 폐암 세포주에서 포도당 운반 단백 유전자의 발현
Tuberculosis and Respiratory Diseases
1998 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-510-017339806